Change of elongation factor 2 in pulmonary hypertension rats and study of interfered with Puerarin
10.13699/j.cnki.1001-6821.2015.07.015
- VernacularTitle:延伸因子2在肺动脉高压大鼠发病中的变化及葛根素的干预作用研究
- Author:
Jun WU
1
;
Liang-Xing WANG
;
Yu-Qiang GONG
;
Meng-Ru SHI
;
Jin-Dan WANG
Author Information
1. 温州医科大学 附属第二医院 重症医学科
- Keywords:
puerarin;
elongation factor 2;
monocrotaline;
pulmonary hypertension;
two-dimensional gel electrophoresis
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):536-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analysis the pulmonary tissue proteomic a-mong the normal , pulmonary hypertensive induced by monocrotaline and Puerarin rats , How the Puerarin inhibits the form of pulmonary hyperten-tion and pneumoangiogram rebuilding cleared.Methods SD ras random-ly divided into 5 groups, the normal group, the solvent group, the model group in two times, monocrotaline and puerarin group ( experimental group ) , 10 in each group .The solvent group was intraperitoneal injected of ethanol mixed with 0.9% sodium chloride solution ( 50 mg · kg -1 weight ) every morning and bred three weeks .The model group was intra-peritoneal injected monocrotaline (50 mg· kg -1 weight)once and respec-tively bred 2 or 3 weeks.The experimental group, after the injection of monocrotaline solution once , was intraperitoneal injected puerarin liquid (40 mg· kg -1 weight ) every morning in three weeks .Set up the pulmo-nary hypertensive rat model sucessfully , two dimensional gel electropho-resis of the total proteins from these five group , gel enzymolysis.Matrix-assisted laser desorption ionization -time of flight mass spectrometry ( MALDI-TOF-MS) and retrieval databank in NCBInr and discuss the results.Results The pulmonary hypertention , pneumoangiogram rebuil-ding , the right ventricle hypertrophy was inhibited by puerarin.The MALDI-TOF-TOF-MS discovered that the elongation factor 2 express very little in normal group and express a great quantity in 3-week-monocrotaline group , but in puerarin group ex-press down quickly.Conclusion The puerarin could inhibit the form of pulmonary hypertention by interfering in elon-gation factor 2 , it worth to study more about it.
