Effect of Tripterygium wilfordii polyglycosidum on connective tissue growth factor in liver in diabetic nephropathy rats
10.13699/j.cnki.1001-6821.2014.07.014
- VernacularTitle:雷公藤多苷对糖尿病肾病大鼠肾脏结缔组织生长因子表达的影响
- Author:
Guo-Ling LIU
1
;
Kun LU
;
Li-Ju YOU
;
Hui GUO
;
Yi-Chuan LI
Author Information
1. 商丘医学高等专科学校 生物化学与分子生物学教研室
- Keywords:
Tripterygium wilfordii polyglycoside;
diabetic nephropathy;
connective tissue growth factor;
glomerular;
immunohistochemical assay
- From:
The Chinese Journal of Clinical Pharmacology
2014;(7):603-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Tripterygium wilfordii polyglycosidum ( TWP ) on the expression and excretion of connective tissue growth factor ( CTGF ).Methods The diabetes mellitus Sprague Dawley rats was induced by streptozotocin .The diabetic rats were random-ly divided in to five groups:normal group ,diabetic model group and three doses (45,90,180 mg · kg -1 ) of TWP groups.Blood glucose ( BG), body weight ( BW) , kidney weight ( KW) , KW/BW, 24 hours urinary al-bumin excretion rate ( UAER ) , blood urea nitrogen ( BUN ) , creatinine clearance ( Ccr) were measured at the end of the 8th week.The patholo-gy changing of the rat kidney was observed by HE staining , and the ex-pression of CTGF was determined by immunohistochemical assay.Re-sults Compared with model group , TWP can significantly improve the general situation of DN rats.TWP (180 mg· kg -1 ) can significantly de-creased fasting BG ( P<0.01 ).TWP ( 90 and 180 mg· kg -1 ) can sig-nificantly increase BW of rats ( P <0.01 ) , and TWP ( 90 and 180 mg · kg -1 ) also can significantly reduce KD , KW/BW, 24hUAER, BUN ( P<0.01 ).And the coloring of the renal tissue mitigate signifi-cantly, the expression of CTGF significantly decreased in TWP (90 and 180 mg· kg -1 ) groups; glomerular hypertrophy , mesangial cellproliferation and glomerular lesions all significantly reduce.Conclusion The TWP can obviously improve the renal function, and dose-related, and its mechanism may be correlated with inhibition on expression of CTGF in kindey.
