Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues
10.12025/j.issn.1008-6358.2025.20241213
- VernacularTitle:血液透析对外周组织中氧代二十碳四烯酸生物转化的影响
- Author:
Tong LIU
1
;
Gollasch MAIK
2
;
C. Luft FRIEDRICH
3
;
Pan LIN
1
;
Jun JI
4
;
Yao MENG
4
Author Information
1. Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, China;Department of Nephrology, Shanghai Geriatrics Medical Center, Shanghai 201100, China.
2. Department of Internal Medicine and Geriatrics, University Medicine Greifswald, Greifswald 17475, Germany.
3. Experimental and Clinical Research Center, a joint institution of Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, Berlin 13125, Germany.
4. Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
- Publication Type:Shortarticle
- Keywords:
hemodialysis;
eicosanoids;
oxo-eicosatetraenoic acid;
biotransformation;
arteriovenous difference
- From:
Chinese Journal of Clinical Medicine
2025;32(1):93-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. Methods Arterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD treatment at Charité – Universitätsmedizin Berlin, Germany, from June to December 2020 were collected. The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results Neither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD. HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma. HD reduced the arteriovenous differences of esterified 12-oxo-ETE, free 15-oxo-ETE, and free 5-oxo-ETE in plasma, while raised the arteriovenous differences of esterified 15-oxo-ETE. Conclusions The oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment, whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment, potentially contributing to the cardiovascular disease.
