Effects of serum containing Sanchong tongluo sanjie formula on the proliferation and apoptosis of Lewis lung cancer cells and its mechanism
- VernacularTitle:三虫通络散结方含药血清对Lewis肺癌细胞增殖和凋亡的影响及机制
- Author:
Yang LI
1
;
Lei CHEN
2
;
Qiachun ZHANG
3
;
Jing ZHANG
1
;
Jingyu FENG
1
;
Qi LIANG
4
Author Information
1. Dept. of Oncology，the Seventh Clinical College of Guangzhou University of Chinese Medicine，Guangdong Shenzhen 518133，China
2. Dept. of Pathology，the Seventh Clinical College of Guangzhou University of Chinese Medicine，Guangdong Shenzhen 518133，China
3. Dept. of Emergency，the Seventh Clinical College of Guangzhou University of Chinese Medicine，Guangdong Shenzhen 518133，China
4. Dept. of Pharmacy，the Seventh Clinical College of Guangzhou University of Chinese Medicine，Guangdong Shenzhen 518133，China
- Publication Type:Journal Article
- Keywords:
Sanchong tongluo sanjie formula;
Lewis lung cancer cells;
proliferation;
invasion;
metastasis;
apoptosis;
PHD2/
- From:
China Pharmacy
2025;36(4):440-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effects of Sanchong tongluo sanjie formula on the proliferation and apoptosis of Lewis lung cancer cells. METHODS The rats were given Sanchong tongluo sanjie formula powder [0.946 g/（kg·d）]， Sanchong tongluo sanjie formula decoction [2.730 g/（kg·d）]， and normal saline intragastrically， and injected with Cisplatin injection （4.2 mg/kg） intra-peritoneally to prepare powder-containing serum， decoction-containing serum， positive control serum and negative control serum. Lewis lung cancer cells were divided into negative control serum group， positive control serum group， and 5%， 10%， 20% drug-containing serum groups. The cell proliferation inhibition rates at 24， 48， and 72 hours post- intervention were measured to screen the optimal intervention concentrations of powder-containing serum and decoction-containing serum. The cell invasion ability， metastasis ability and apoptotic rate were detected in the negative control serum group， positive control serum group， 20% powder-containing serum group， and 20% decoction-containing serum group. Protein expressions of vascular endothelial growth factor （VEGF）， hypoxia-inducible factor-1α （HIF-1α）， prolyl hydroxylase-2 （PHD2）， matrix metalloproteinase-2 （MMP-2）， extracellular regulated protein kinases （ERK），c-Jun N-terminal kinase （JNK） and p38 mitogen-activated protein kinase （p38 MAPK） were detected. RESULTS The cell proliferation inhibition rates for both the 20% powder-containing serum group and the 20% decoction-containing serum group， when intervened for 48 hours， were not less than 50%. Compared with negative control serum group， the number of invasive Lewis lung cancer cells and migration distance were decreased significantly， while the apoptotic rate was increased significantly （P＜0.05）； the apoptotic rate in the 20% powder- containing serum group was significantly higher than 20% decoction-containing serum group （P＜0.05）. The protein expressions of PHD2 and p38 MAPK were increased significantly in the 20% powder-containing serum group （P＜0.05）， while the protein expression of HIF-1α was decreased significantly in the 20% decoction-containing serum group （P＜0.05）. CONCLUSIONS Sanchong tongluo sanjie formula can inhibit the proliferation， invasion and metastasis of Lewis lung cancer cells while promoting their apoptosis. The mechanism of action may be related to regulating the PHD2/HIF-1α signaling pathway. Furthermore， the powder demonstrates superior efficacy compared to the decoction， suggesting that they may possess different mechanisms of action.
