Study on the effect of berberine combined with fluconazole on fluconazole-tolerant Candida albcians strains
10.12206/j.issn.2097-2024.202409047
- VernacularTitle:小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究
- Author:
Zecheng SONG
1
;
Shanshan MA
1
;
Qiaoling HU
2
;
Hua ZHONG
1
;
Yan WANG
3
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China;School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
3. School of Pharmacy, Naval Medical University, Shanghai 200433, China;The Center for Fungal Infectious Diseases Basic Research and Innovation of Medicine and Pharmacy, Ministry of Education, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
Candida albicans;
berberine;
fluconazole;
drug combination;
drug tolerance
- From:
Journal of Pharmaceutical Practice and Service
2025;43(2):87-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the combined effect of berberine （BBR） and fluconazole （FLC） on FLC-tolerant Candida albicans in vitro. Methods The sensitivity of 8 strains of Candida albicans to FLC was assessed by determining their minimal inhibitory concentration （MIC） using broth microdilution method. FLC-tolerant strains were screened from FLC-sensitive strains by disk diffusion assay. The effect of BBR combined with FLC on FLC-tolerant Candida albicans was investigated by disk diffusion assay. Results All eight strains of Candida albicans exhibited sensitivity to FLC, with minimal inhibitory concentration （MIC50） values below 0.5 μg/ml. Strains Y0109, 9821, 7879, 7654, and 9296 displayed colony growth in the inhibition zone after 48 h of constant temperature incubation, indicating FLC tolerance. When strains Y0109 and 9821 were subjected to a combination of BBR and FLC, the number of colonies within the inhibition zone decreased progressively with the increase of BBR concentration following a 48 h constant temperature culture. The inhibition zone became clear with the increasing of BBR concentration and increased with the increase of FLC loading, which showed a dose-dependent relationship. Conclusion The BBR combined with FLC demonstrated efficacy against FLC-tolerant strains.
