Effects of imperatorin on malignant biological behavior of gastric cancer cells by regulating ThPOK expression
- VernacularTitle:欧前胡素调节ThPOK表达对胃癌细胞恶性生物学行为的影响
- Author:
Lan CHEN
1
;
Lingli XIA
1
;
Ying CHEN
1
;
Gang ZHANG
1
;
Feng WEN
2
Author Information
1. Dept. of Gastroenterology，Wuhan Sixth Hospital Affiliated to Jianghan University，Wuhan 430015，China
2. Dept. of Gastroenterology，Central Theater Command General Hospital，Wuhan 430012，China
- Publication Type:Journal Article
- Keywords:
imperatorin;
ThPOK;
gastric cancer cells;
immune escape;
malignant biological behavior
- From:
China Pharmacy
2025;36(2):191-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of imperatorin （IMP-SD） on malignant biological behavior of gastric cancer （GC） cells by regulating zinc finger and BTB domain 7B （ThPOK）. METHODS Human GC cells MKN-7 were used as the research object and then divided into control group （no treatment）， IMP-SD low-， medium- and high-concentration groups （40， 80 and 160 μmol/L IMP-SD）， si-ThPOK and si-NC group [treated with 160 μmol/L IMP-SD and then transfected with ThPOK small interfering RNA （si-ThPOK） or its negative control （si-NC）]. After treatment， cell clone formation， migration and invasion abilities and apoptosis of MKN-7 cells were detected； the killing activity of NK cells， T cells classification， the protein expressions of ThPOK， programmed death-1 （PD-1） and programmed death-ligand 1 （PD-L1） were all determined. RESULTS Compared with the control group， the number of cell clones， migration number， invasion number， and the protein expressions of PD-1 and PD-L1 were decreased or down-regulated significantly in IMP-SD groups， while the cell apoptotic rate， NK cell killing activity， CD4+ T proportion， the ratio of CD4+ T proportion and CD8+ T proportion （CD4+ T/CD8+ T）， and the protein expression of ThPOK were increased or up-regulated significantly， in a concentration-dependent manner （P＜0.05）. Compared with IMP-SD high-concentration group and si-NC group， the number of cell clones， migration number， invasion number， and the protein expressions of PD-1 and PD-L1 were increased or up-regulated significantly in si-ThPOK group， while the cell apoptotic rate， NK cell killing activity， CD4+ T proportion， CD4+ T/CD8+ T， and the protein expression of ThPOK were decreased or down-regulated significantly （P＜0.05）. CONCLUSIONS IMP-SD may reduce the clonal formation， migration and invasion abilities of GC cells， promote their apoptosis and inhibit their immune escape by promoting ThPOK expression.
