Effects of imperatorin on malignant biological behavior of gastric cancer cells by regulating ThPOK expression
10.6039/j.issn.1001-0408.2025.02.10
- VernacularTitle:欧前胡素调节ThPOK表达对胃癌细胞恶性生物学行为的影响
- Author:
Lan CHEN
1
;
Lingli XIA
;
Ying CHEN
;
Gang ZHANG
;
Feng WEN
Author Information
1. 江汉大学附属武汉市第六医院消化内科,武汉 430015
- Publication Type:Journal Article
- Keywords:
imperatorin;
ThPOK;
gastric cancer cells;
immune escape;
malignant biological behavior
- From:
China Pharmacy
2025;36(2):191-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of imperatorin(IMP-SD)on malignant biological behavior of gastric cancer(GC)cells by regulating zinc finger and BTB domain 7B(ThPOK).METHODS Human GC cells MKN-7 were used as the research object and then divided into control group(no treatment),IMP-SD low-,medium-and high-concentration groups(40,80 and 160 μmol/L IMP-SD),si-ThPOK and si-NC group[treated with 160 μmol/L IMP-SD and then transfected with ThPOK small interfering RNA(si-ThPOK)or its negative control(si-NC)].After treatment,cell clone formation,migration and invasion abilities and apoptosis of MKN-7 cells were detected;the killing activity of NK cells,T cells classification,the protein expressions of ThPOK,programmed death-1(PD-1)and programmed death-ligand 1(PD-L1)were all determined.RESULTS Compared with the control group,the number of cell clones,migration number,invasion number,and the protein expressions of PD-1 and PD-L1 were decreased or down-regulated significantly in IMP-SD groups,while the cell apoptotic rate,NK cell killing activity,CD4+T proportion,the ratio of CD4+T proportion and CD8+T proportion(CD4+T/CD8+T),and the protein expression of ThPOK were increased or up-regulated significantly,in a concentration-dependent manner(P＜0.05).Compared with IMP-SD high-concentration group and si-NC group,the number of cell clones,migration number,invasion number,and the protein expressions of PD-1 and PD-L1 were increased or up-regulated significantly in si-ThPOK group,while the cell apoptotic rate,NK cell killing activity,CD4+T proportion,CD4+T/CD8+T,and the protein expression of ThPOK were decreased or down-regulated significantly(P＜0.05).CONCLUSIONS IMP-SD may reduce the clonal formation,migration and invasion abilities of GC cells,promote their apoptosis and inhibit their immune escape by promoting ThPOK expression.
