Combined effect of bisphosphonate and recombinant human bone morphogenetic protein 2 on bone healing of rat calvarial defects.
10.1186/s40902-015-0015-3
- Author:
Ho Chul KIM
1
;
Jae Min SONG
;
Chang Joo KIM
;
Sang Yong YOON
;
In Ryoung KIM
;
Bong Soo PARK
;
Sang Hun SHIN
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, 626-787 Yangsan, Mulgeum-eup, Korea. ssh8080@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Bisphophonate;
Bone morphogenetic protein 2;
Bone regeneration;
Histology;
Alendronate
- MeSH:
Alendronate;
Animals;
Bone Morphogenetic Protein 2*;
Bone Regeneration;
Collagen Type I;
Humans;
Immunohistochemistry;
Osteoblasts;
Osteogenesis;
Osteoprotegerin;
RANK Ligand;
Rats*;
Skull;
Transplants
- From:Maxillofacial Plastic and Reconstructive Surgery
2015;37(6):16-
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aimed to investigate new bone formation using recombinant human bone morphogenetic protein 2 (rhBMP-2) and locally applied bisphosphonate in rat calvarial defects. METHODS: Thirty-six rats were studied. Two circular 5 mm diameter bony defect were formed in the calvaria using a trephine bur. The bony defect were grafted with Bio-Oss(R) only (group 1, n = 9), Bio-Oss(R) wetted with rhBMP-2 (group 2, n = 9), Bio-Oss(R) wetted with rhBMP-2 and 1 mM alendronate (group 3, n = 9) and Bio-Oss(R) wetted with rhBMP-2 and 10 mM alendronate (group 4, n = 9). In each group, three animals were euthanized at 2, 4 and 8 weeks after surgery, respectively. The specimens were then analyzed by histology, histomorphometry and immunohistochemistry analysis. RESULTS: There were significant decrease of bone formation area (p < 0.05) between group 4 and group 2, 3. Group 3 showed increase of new bone formation compared to group 2. In immunohistochemistry, collagen type I and osteoprotegerin (OPG) didn't show any difference. However, receptor activator of nuclear factor kappaB ligand (RANKL) decreased with time dependent except group 4. CONCLUSION: Low concentration bisphosphonate and rhBMP-2 have synergic effect on bone regeneration and this is result from the decreased activity of RANKL of osteoblast.
