Study on the Analgesic Material Basis of Zhejiang Vinegar Corydalis Rhizoma Based on Chemometrics
10.13748/j.cnki.issn1007-7693.20240769
- VernacularTitle:基于化学计量学的浙产醋延胡索镇痛的物质基础研究
- Author:
LI Yafei
1
;
ZHAO Mingling
1
;
TANG Rui
1
;
FANG Ke'er
1
;
ZHANG Hair
1
;
GE Weihong
2
;
DU Weifeng
2
Author Information
1. Zhejiang Chinese Medical University School of Pharmaceutical Sciences Zhejiang Chinese Medical University Research Center of TCM Processing Technology, Hangzhou 311402, China
2. Zhejiang Chinese Medical University School of Pharmaceutical Sciences Zhejiang Chinese Medical University Research Center of TCM Processing Technology, Hangzhou 311402, China Zhejiang Chinese Medical University Chinese Medicine Yinpian Co., Ltd., Hangzhou 311401, China
- Publication Type:Journal Article
- Keywords:
vinegar Corydalis Rhizoma;
fingerprint;
analgesia;
chemometrics;
material basis
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(14):1892-1899
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To explore the correlation between HPLC fingerprint and analgesic efficacy of Zhejiang vinegar Corydalis Rhizoma, and to screen out the material basis of analgesic effect of Zhejiang vinegar Corydalis Rhizoma.METHODS Established HPLC fingerprint of Zhejiang vinegar Corydalis Rhizoma and dysmenorrhea model in rats. The weight loss rate, writhing response, serum malondialdehyde(MDA), estradiol(E2), prostaglandin E2(PGE2) and prostaglandin F2α(PGF2α) levels were used as the evaluation indexes of analgesic efficacy. Combined with chemometrics, the main active components of analgesic effect of Zhejiang vinegar Corydalis Rhizoma and the important components with great contribution to the content were screened.RESULTS The fingerprints of different batches of Zhejiang vinegar Corydalis Rhizoma were established, and nine components were identified; compared with the blank group, the weight loss rate, writhing reaction, MDA, E2 and PGF2α levels in the model group were significantly increased. Compared with the model group, the above indexs in the administration group was decreased. Compared with the blank group, the PGE2 level in the model group was significantly decreased. Compared with the model group, the PGE2 level in the administration group was significantly increased. The vinegar Corydalis Rhizoma produced in Zhejiang had a good analgesic effect, and the main active components of its analgesic effect were protopine, palmatine hydrochloride, and dehydrocorydaline. The important components with greater contribution to the content were tetrahydropalmatine, protopine, palmatine hydrochloride and stylopine.CONCLUSION The efficacy of Zhejiang vinegar Corydalis Rhizoma is the result of the combined action of multiple components, and each component has a strong correlation with the pharmacodynamic indexes. This study provides a reference for the screening of analgesic material basis of Zhejiang vinegar Corydalis Rhizoma.
