Effect of antibiotics on gut microbiota and cognitive behaviors in rats
10.19405/j.cnki.issn1000-1492.2022.09.020
- Author:
Yanli Li
1
;
Xiaorong Yang
2
;
Ce Zhang
2
;
Junhong Guo
1
Author Information
1. Dept ofNeurology, First Hospital of Shanxi Medical University, Taiyuan 030001
2. Dept ofPhysiology, Key Laboratory of Cellular Physiology,Ministry of Education ,Shanxi Medical University, Taiyuan 030001
- Publication Type:Journal Article
- Keywords:
antibiotics;
gut microbiota;
cognitive behaviors;
DMTP;
Y maze
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1447-1452
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the changes and recovery of gut microbiota in rats after broad-spectrum antibiotic exposure and its effect on cognitive behaviors.
Methods :Rats were randomly divided into antibiotic(AT) group and control group.AT group was treated with antibiotic cocktail containing ampicillin, ancomycin, metronidazole and imipenem for 3 days, and control group was gavaged with the same amount of normal saline.On the 4 th day(24 h after the last antibiotic treatment) and 2 months, feces were collected for 16 S rRNA gene sequencing to detect the changes of gut microbiota; At 2 months, the cognitive behaviors were detected by operant-based delayed matching to position(DMTP) task and Y maze.
Results :On day 4,the gut microbiota in AT group decreased, including a decrease in microbiota diversity(P<0.000 1),a reduction ofBacteroidetes(P<0.000 1) andFirmicutes(P=0.000 1),and an increase ofProteobacteria(P<0.000 1).After discontinuation of antibiotics, the microbiota began to recover, which had no difference from control group at 2 months.And there was no significant difference in cognitive behaviors between AT group and control group.
Conclusion:The broad-spectrum antibiotics can decrease gut microbiota in rats.However, this decrease is temporary.The microbiota returns to normal at 2 months after discontinuation of antibiotics.And it does not affect the cognitive behaviors of rats.
- Full text:2024122711130776784抗生素对大鼠肠道菌群和认知行为的影响_李艳丽.pdf
