Comparative study on the effect of BoneCeramic and Bio-Oss on promoting osteogenesis after PAOO in rats and osteogenic differentiation of bone marrow stromal cells
10.19405/j.cnki.issn1000-1492.2022.09.017
- Author:
Weiqiong Li
1
;
Yuqing Zhang
1
;
Jianguang Xu
1
;
Hongyan Zhang
1
Author Information
1. Stomatological College of Anhui Medical University, The Afiliated Stomatological Hospital of Anhui Medical University , Anhui Province Key Laboratory of Oral Diseases Research , Hefei 230032
- Publication Type:Journal Article
- Keywords:
BoneCeramic;
Bio⁃Oss;
periodontally accelerated osteogenic orthodontics;
bone marrow mesenchymal stem cells;
osteogenic
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1430-1435
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To compare the effect of BoneCeramic and Bio-OSS on osteogenic after PAOO and bone marrow stromal osteogenic differentiation in rats.
Methods:36 8-week-old SD rats were enrolled and divided into 3 groups: corticotomy group(CO group);corticotomy+Bio-Oss implantation group(BO group);corticotomy+BoneCeramic implantation group(BC group).Immediately, a standard orthodontic spring(50 N/kg) was activated to pull the right upper first molars toward midrange movement.At 28,60 days after activation, 6 rats were randomly selected from each group and sacrificed.The mesial movement distance, palatal alveolar bone height loss(ABHL) and bone volume fraction(BVF) of the first molars were measured by micro-CT.The
Results :There was no difference in tooth movement between the three groups; The ABHL value of the control group was higher than that of the experimental group at 28 and 60 days, and the BVF value was lower than that of the two groups.Meanwhile, the ABHL value of the BO group was higher than that of the BC group at 60 days, and the BVF value was lower than that of the BC group.CCK-8 showed that the two materials did not inhibit the growth of BMSCs.SEM showed that BMSCs were more distributed on the surface of BoneCeramic material.Quantitative ALP detection showed that BMSCs exhibited higher ALP activity on the surface of BoneCaremic material, and mRNA expressions of RUNX-2,OPN and OCN were higher.
Conclusion : BoneCeramic is more beneficial to osteogenesis after PAOO and osteogenic differentiation of BMSCs in SD rats.
- Full text:2024122617521659486BoneCeramic与B...细胞成骨分化效果的比较研究_李伟琼.pdf
