Involvement of locus coeruleus in the regulation of depression-induced chronic pain behavior
10.19405/j.cnki.issn1000-1492.2024.09.004
- VernacularTitle:蓝斑核参与抑郁导致的慢性疼痛行为的调控
- Author:
Xi YUAN
1
;
Yuqing LIU
;
Qian MENG
Author Information
1. 安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,抗炎免疫药物安徽省协同创新中心,合肥 230032
- Keywords:
locus coeruleus;
chemogenetics;
chronic pain;
chronic restraint stress;
electrophysiology in vitro;
novelty-suppressed feeding test;
forced swimming test
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1517-1524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether the locus coeruleus(LC)mediates the pain in mice with depressive-like behaviors and underlying mechanisms.Methods Male C57BL/6J mice were randomly divided into two groups,in-cluding control and chronic restraint stress(CRS)group.Novelty-suppressed feeding test,tail suspension test and forced swimming test were used to assess the formation of depressive-like behaviors and the establishment of CRS model.The mechanical pain threshold of mice was detected by Von Frey brush test.LC neuronal activity was de-tected by c-Fos immunofluorescence staining.Neuronal excitability in LC was detected using electrophysiology of i-solated brain slices.The influence on pain and depressive-like behaviors was identified by chemogenetics in LC.Results Compared with the Control group,CRS mice displayed significant depressive-like behavior after 3 weeks of CRS(CRS 3W),and the model was successful;CRS 3W mice showed a significant decrease in mechanical pain threshold and developed mechanical pain sensitivity.A lot of c-Fos+cells of LC was observed in CRS 3W mice,90％ of them were noradrenergic neurons.Compared with the Control group,the neuronal excitability of noradren-ergic neurons decreased in LC of CRS 3W mice.Compared with the Control group,chemogenetic activation of the LC noradrenergic neurons significantly reduced mechanical pain sensitization in CRS 3W mice.Conclusion The excitability of noradrenergic neurons in LC of CRS 3W mice decreases,and chemogenetic activation of noradrener-gic neurons in LC can relieve pain sensitization in CRS 3W mice.
- Full text:2024122416065542667蓝斑核参与抑郁导致的慢性疼痛行为的调控_袁溪.pdf
