The protective effect of a traditional chinese medicine composition on ANIT induced liver injury in mice with cholestasis
10.12206/j.issn.2097-2024.202305008
- VernacularTitle:一种中药组合物对ANIT诱导的小鼠胆汁淤积肝损伤的保护作用研究
- Author:
Nian YANG
1
;
Bole ZHANG
1
;
Junxia ZHANG
1
;
Zhenqiang ZHANG
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China.
- Publication Type:Originalarticles
- Keywords:
Traditional chinese medicine composition;
Cholestasis;
Biochemical indexes;
Pathological changes
- From:
Journal of Pharmaceutical Practice and Service
2024;42(12):508-511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of a traditional chinese medicine composition on ANIT induced cholestasis liver injury in mice. Methods 8-week old SPF Kunming mice were randomly divided into 6 groups, 10 mice in each group which were the blank control group （normal saline gavage）, the model control group （normal saline gavage）, the low （gavage dose: 5.25 g/kg） group, the medium （gavage dose: 11.25 g/kg） group and the high （gavage dose: 20.25 g/kg） group of traditional chinese medicine composition, the positive control （Ursodeoxycholic acid, UDCA, 0.1 g/kg）. Mice were administered with continuous gavage once a day, for 7 consecutive days. On the 5th day, the blank normal control group was given an equal amount of olive oil by gavage, all other groups were given ANIT （65 mg/kg） by gavage for modeling. After 48 hours of modeling, 30 minutes after the last administration of each group of mice, blood and liver tissue samples were collected for testing of various indicators. The corresponding kits were used to detect the content of alanine transaminase （ALT）, aspartate transaminase （AST）, alkaline phosphatase （ALP）, total bile acid （TBA）, γ-glutamyl transpeptidase （γ-GT）, total bilirubin （TBIL）, superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX） and malondialdehyde （MDA） in peripheral blood of mice, respectively. HE staining was used to observe the pathological changes in the liver of mice. Results Compared with the model group, each dose group of traditional chinese medicine composition reduced the levels of ALT, AST, ALP, TBA, TBIL, γ-GT and MDA （P<0.05） significantly, markedly increased the levels of SOD and GSH-PX （P<0.05）. Compared with the model group, the low-dose and high-dose groups of traditional chinese medicine composition showed a decrease in hepatic steatosis and a reduction in inflammatory. The moderate dose group showed a significant reduction in hepatic cell steatosis and a significant improvement in inflammatory infiltration, the liver cells were arranged in cords, and the structure of lobules of liver was intact. Conclusion Traditional chinese medicine composition could improve serum biochemical indicators of liver injury, reduce jaundice and eliminate oxidative free radicals, thereby exerting a protective effect against ANIT-induced cholestatic liver injury in mice.
