Tnfrsf11a Cre mediates YFP labeling some of tissue macrophages
10.19405/j.cnki.issn1000-1492.2022.09.001
- Author:
Fengjiao Yang
1
;
Ziwei Huang
1
;
Dianyuan Zhao
2
;
Long Xu
1
;
Li Tang
1
Author Information
1. Dept ofImmunology,School of Basic Medical Sciences ,Anhui Medical University,Hefei 230032
2. Institute of Lifeomics ,Academy ofMilitary Medical Sciences ,Academy ofMilitary Sciences ,Beijing 102206; State Key Laboratory of Proteomics ,Beijing Proteome Research Center,National Center for Protein Sciences ,Beijing 102206
- Publication Type:Journal Article
- Keywords:
Tnfrsf11aCre;
Rosa26yfp;
tissue macrophages;
brain microglia;
cre recombinase;
reporter mouse;
flow cytometry
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1345-1349
- CountryChina
- Language:Chinese
-
Abstract:
Objective : Tnfrsf11 a Cre Rosa26 yfp reporter gene mice were prepared to determine the efficiency of Cre-mediated recombination using flow cytometry in different tissue-resident macrophages.
Methods :TheTnfrsf11 a Cre Rosa26 yfp reporter mice were generated by crossingTnfrsf11 a Cre mice withRosa26 yfp mice and identified by PCR.Brain microglia, liver macrophages, kidney macrophages, alveolar macrophages and spleen macrophages were separated from adultTnfrsf11 a Cre Rosa26 yfp reporter mouse and yellow fluorescent protein(YFP) labeling efficiency was analyzed by flow cytometry.
Results : YFP expression percentage was about 91.27% of brain microglia inTnfrsf11 a Cre Rosa26 yfp reporter mice, but liver, spleen, and alveolar macrophages were inefficiently targeted(63.60%,69.66%,32.76%).
Conclusion:Tnfrsf11 a Cre mice were qualified as conditional knockout model mice of brain microglia andTnfrsf11 a Cre Rosa26 yfp reporter mice can be used as a tool for conditional knockout of brain microgliain vivo.
- Full text:2024122410532095422Tnfrsf11a~(Cr...素蛋白有效标记脑小胶质细胞_杨凤娇.pdf
