Immunoinformatics approach for design novel multiepitope prophylactic and therapeutic vaccine based on capsid proteins L1 and L2 and oncoproteins E6 and E7 of human papillomavirus 16 and human papillomavirus 18 against cervical cancer
10.24171/j.phrp.2024.0013
- Author:
Nicholas RYAN
1
;
Sari Eka PRATIWI
;
Mardhia MARDHIA
;
Ysrafil YSRAFIL
;
Delima Fajar LIANA
;
Mahyarudin MAHYARUDIN
Author Information
1. Medical Study Program, Faculty of Medicine, Universitas Tanjungpura, Pontianak, Indonesia
- Publication Type:Original Article
- From:
Osong Public Health and Research Perspectives
2024;15(4):307-328
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objectives:This study aimed to identify the optimal protein construction for designing a multi-epitope vaccine with both prophylactic and therapeutic effects against cervical cancer, utilizing an immunoinformatics approach. The construction process involved using capsid epitopes L1 and L2, as well as oncoproteins E5, E6, and E7 from human papillomavirus (HPV) types 16 and 18.
Methods:An experimental in silico analysis with an immunoinformatics approach was used todevelop 2 multi-epitope vaccine constructs (A and B). Further analysis was then conducted tocompare the constructs and select the one with the highest potential against cervical cancer.
Results:This study produced 2 antigenic, non-allergenic, and nontoxic multi-epitope vaccine constructs (A and B), which exhibited the ideal physicochemical properties for a vaccine.Further analysis revealed that construct B effectively induced both cellular and humoralimmune responses.
Conclusion:The multi-epitope vaccine construct B for HPV 16 and 18, designed for both prophylactic and therapeutic purposes, met the development criteria for a cervical cancer vaccine. However, these findings need to be validated through in vitro and in vivo experiments.
