Simultaneous Determination of 8 Anti-infective Drugs in Anti-dandruff Hair Products by QuEChERS-liquid Chromatography Tandem Mass Spectrometry
10.13748/j.cnki.issn1007-7693.20223710
- VernacularTitle:QuECHERS-液相色谱-串联质谱同时测定去屑类发用产品中8种抗感染药物
- Author:
Cen CHEN
1
;
Yanlin GAO
2
;
Linqi YAN
1
;
Qianyuan CHENG
3
Author Information
1. Zhejiang Institute for Food and Drug Control, NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetics, Hangzhou 310052, China;
2. Zhejiang Chinese Medical University, Hangzhou 310053, China
3. Zhejiang Institute for Food and Drug Control, NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetics, Hangzhou 310052, China;Zhejiang Chinese Medical University, Hangzhou 310053, China
- Publication Type:Journal Article
- Keywords:
QuECHERS / HPLC-MS/MS;
anti-infective drugs;
anti-dandruff hair products
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(23):3275-3280
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a QuECHERS-liquid chromatography-tandem mass spectrometry method for simultaneous determination of 3 triazole antifungal drugs including itraconazole, voriconazole, posaconazole, and 5 nitroimidazoles including ronidazole, dimetridazole, secnidazole, tinidazole and ornidazole. METHODS The sample was added into 1 mL of saturated sodium chloride solution and extracted with acetonitrile, and then purified by QuECHERS method. The separation was performed on a ZORBAX SB-C18 column. The mobile phase consisted of 0.1% formic acid acetonitrile-0.1% formic acid aqueous solution by gradient elution, electrospray ionization(ESI+) source was applied and operated in the positive mode. Multiple reaction monitoring mode was used to monitor ions. Matrix-matched calibration was used to quantitative calculation. RESULTS Eight anti-infective drugs showed good linear relationship in the range of 20.0-1 000.0 ng·mL-1. The linear correlation coefficients of the method were ＞0.995. At three levels, the average recoveries ranged from 90.3% to 113.0%, with the RSD(n=6) from 0.4% to 2.6%. The limits of quantification of the method were among 0.056-1.89 μg·g-1, and the limits of detection were among 0.014-0.56 μg·g-1. CONCLUSION This method is rapid, accurate and has good purification effect, which can be applied to the detection of 8 anti-infective drugs in anti-dandruff hair products.
