Brigatinib enhanced radiation sensitivity of colorectal cancer cells by inhibiting EGFR phosphorylation
10.19405/j.cnki.issn1000-1492.2022.02.010
- Author:
Guanshuo Hu
1
;
Guoping Zhao
2
Author Information
1. Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes ofPhysical Science , Chinese Academy of Sciences , Hefei 230031 ; Science Island Branch of Graduate School , University of Science and Technology of China , Hefei 230026
2. Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes ofPhysical Science , Chinese Academy of Sciences , Hefei 230031
- Publication Type:Journal Article
- Keywords:
colorectal cancer cells LOVO;
Brigatinib;
EGFR;
p ⁃EGFR
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):212-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of Brigatinib on radiation sensitivity of colorectal cancer LOVO cells by treating colorectal cancer LOVO cells with a combination of radiation and brigatinib.
Methods :Western blot and immunofluorescence detection were used to detect the amount of epidermal growth factor receptor(EGFR) , p ⁃ EGFR expression in colorectal cancer cells after treatment with different irradiation doses. The expression levels of EGFR , p ⁃EGFR , γ⁃H2AX , cleaved caspase⁃3 , cleaved caspase⁃7 and cleaved caspase⁃9 in LOVO cells were determined by Western blot , the levels of Non⁃homologous End Joining⁃NHEJ were detected by DR⁃GFP plasmid system , cell viability was detected by CCK⁃8 and apoptosis was detected by annexinv FITC / PI double labeling.
Results :Radiation treatment significantly increased EGFR phosphorylation in LOVO cells , which was inhibited in a
dose⁃dependent manner after treatment with Brigatinib. Non⁃homologous End Joining repair was inhibited following Brigatinib treatment , and a significant increase in apoptosis was observed in LOVO cells treated with combined Brigatinib and radiation.
Conclusion :Brigatinib inhibited non homologous end joining repair and promoted apoptosis in LOVO cells by inhibiting EGFR phosphorylation , which eventually increased the radiation sensitivity of LOVO cells.
- Full text:2024120516262297510伯格替尼通过抑制EGFR磷...强结直肠癌细胞的辐射敏感性_胡关朔.pdf
