Relationship between RAS expression and liver function inj ury in endotoxemia model rats
10.19405/j.cnki.issn1000-1492.2022.01.022
- Author:
Jingling Li
1
;
Chenghong Yin
2
Author Information
1. Dept of Medicine , Tsinghua University Hospital ,Beijing 100086
2. Beijing Obstetrics and Gynecology Hospital , Beijing 100026
- Publication Type:Journal Article
- Keywords:
endotoxemia;
liver;
renin⁃angiotensin system;
inflammatory response;
stress response
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):116-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the changes of liver renin⁃angiotensin (RAS) system as well as its relationship with liver function in endotoxemia rats.
Methods :Totally 90 rats were randomly divided into control group and 2 , 4 , 8 , 12 , 24 , 48 , 72 h and 7 d after injection group , which were prepared for sepsis model by tail vein injection of LPS except for the control group. Animals in the control group were collected samples at 8 h after injection , while animals in the model group were collected samples at each time points (2 , 4 , 8 , 12 , 24 , 48 , 72 h and 7 d) . Heart blood was collected to measure liver markers alanine amine aminotransferase ( ALT) , aspartate aminotransferase (AST) , lactate dehydrogenase (LDH) , RAS markers renin⁃angiotensin (PRA) , angiotensin converting enzyme (ACE) and angiotensin Ⅱ (Ang Ⅱ ) . Liver tissues were extracted to observe the pathology characteristics and detect PRA , ACE , Ang Ⅱ and angiotensin type 1 receptor ( AT1R) mRNA.
Results :The levels of AST , ALT and LDH in the model group gradually increased after LPS injection , and AST and LDH in the model group were higher than those in the control group at 4 ~ 48 h , ALT in the model group was higher than that in the control group at 2 to 48 h , and the differences were statistically significant (P < 0. 05) . The circle and liver PRA level in the model group increased rapidly after injection , which was higher than that in the control group at 2 to 48 h. The circle and liver levels of ACE and Ang Ⅱ increased after injection too , which were both higher than those in the control group at 2 to 28 h , and the difference was statistically significant ( P < 0. 05 ) . The expression of AT1R mRNA in the model group gradually increased after injection , which was higher than that in the control group at 4 to 48 h , and the difference was statistically significant ( P < 0. 05 ) .
Conclusion :Liver function damage caused by endotoxemia may be related to the activation of local RAS in the liver.
- Full text:2024120310304313396内毒素血症大鼠肝脏RAS表达与肝功能损伤的关系_李晶铃.pdf
