Study on the characteristic branch sites of oligosaccharides of Astragalus polysaccharide APS-Ⅱ enzymolysis based on high resolution mass spectrometry
10.16438/j.0513-4870.2023-1379
- VernacularTitle:基于高分辨质谱技术的黄芪多糖APS-Ⅱ酶解活性寡糖特征分支位点的研究
- Author:
Yu-chong LIU
1
;
Hu-feng LI
1
;
Ke LI
2
;
Xue-mei QIN
1
;
Yu-guang DU
3
;
Zhen-yu LI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China; Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China
2. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China; Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China; Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
- Publication Type:Research Article
- Keywords:
APS-Ⅱ;
enzymolysis;
connection mode;
structural analysis;
high resolution mass spectrometry
- From:
Acta Pharmaceutica Sinica
2024;59(7):2108-2116
- CountryChina
- Language:Chinese
-
Abstract:
italic>Astragalus polysaccharides are the most immunoregulatory active and abundant substances in Astragalus, with anti-tumor, anti-viral, and immune-promoting biological activities. They have been widely used in clinical practice. Previous studies have found that Astragalus polysaccharides are mainly composed of two different polysaccharides, APS-I (>2 000 kDa) and APS-Ⅱ (10 kDa), with APS-Ⅱ (10 kDa) being the most active component of Astragalus polysaccharides. We used α-1,4-glucan endo-hydrolysis enzyme to degrade APS-Ⅱ into oligosaccharides, and screened the immune activity of oligosaccharides in vitro. We found that the overall immune activity of 2-9 oligosaccharides was low, while the immune activity of 10-14 oligosaccharides was strong, and the activity was better than that of untreated APS-Ⅱ. To investigate the key structural features of APS-Ⅱ oligosaccharides that play a role in immune activity, we used MALDI-TOF-MS biochemical mass spectrometry and high-resolution mass spectrometry instrument ESI-Q Exactive-MS to analyze the APS enzymatic oligosaccharides. By comparing, we found that 10-14 oligosaccharides contain 1→4 and 1→6 branched structures with coexisting linkages, suggesting that 1→4 and 1→6 linkages in branched structures are key structural features that play a role in the immune activity of APS-Ⅱ, laying a theoretical foundation for the structure-activity relationship of Astragalus polysaccharides and oligosaccharides.
