Study on quality evaluation of Gegen Qinlian decoction based on UHPLC fingerprint and multi-component quantification
10.16438/j.0513-4870.2024-0201
- VernacularTitle:基于UHPLC指纹图谱和多成分定量的葛根芩连汤质量评价研究
- Author:
Juan XIE
1
;
Qi TANG
2
;
Pan ZHANG
2
;
Xin LI
1
;
Kai-shun BI
3
;
Qing LI
2
Author Information
1. School of Pharmacy, Southern Medical University, Guangzhou 510515, China
2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
3. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Pharmacy, Southern Medical University, Guangzhou 510515, China
- Publication Type:Research Article
- Keywords:
Gegen Qinlian decoction;
fingerprint;
multicomponent quantification;
quality control
- From:
Acta Pharmaceutica Sinica
2024;59(8):2365-2371
- CountryChina
- Language:Chinese
-
Abstract:
Gegen Qinlian decoction has a wide range of clinical applications. However, there is a lack of systematic quality evaluation methods to ensure the safety and effectiveness of Gegen Qinlian decoction in clinical use. The UHPLC fingerprint and multi-component determination method of Gegen Qinlian decoction were established to provide scientific basis for the quality control and evaluation of Gegen Qinlian decoction. The chromatography was performed on a ZORBAX Eclipse Plus-C18 column (150 mm × 4.6 mm, 3.5 μm) with mobile phase consisted of acetonitrile (A) - 20 mmol·L-1 ammonium acetate (containing 0.8% acetic acid and 0.5% triethylamine) (B) and gradient elution at a flow rate of 1.0 mL·min-1. The column temperature was 25 ℃, the detection wavelength was 260 nm, the fingerprint of 10 batches of Gegen Qinlian decoction was determined, and the similarity evaluation system of TCM chromatographic fingerprint was used for comprehensive analysis, and 9 components were quantitatively analyzed. In the fingerprint study of Gegen Qinlian decoction, a total of 18 peaks were obtained, 12 of which were identified by reference substances. Moreover, the similarity of 10 batches of Gegen Qinlian decoction was good, and all of them were greater than 0.99. In the multi-component quantitative analysis, the linear relationship between the nine components and the peak area was good (r ≥ 0.999) in the corresponding mass concentration range. The average recovery rate was 94.4%-100.3%, and the RSD was 0.1%-1.4%. The fingerprint of Gegen Qinlian decoction was studied under the same wavelength, and the content of 9 main components were determined by our established method. The method has high sensitivity and strong specificity, which provided a comprehensive scientific basis for the comprehensive evaluation of the quality of Gegen Qinlian decoction.
