Four new dammarane-type triterpenoid saponins from <italic>Gynostemma pentaphyllum</italic> (Thunb.) Makino

Guang YANG; Hai-zhen LIANG; Jie ZHANG; Xiao-juan CHEN; Bao-lin GUO; Bai-ping MA

Return

Four new dammarane-type triterpenoid saponins from Gynostemma pentaphyllum (Thunb.) Makino

VernacularTitle:绞股蓝中4个新达玛烷型三萜皂苷的分离鉴定
Author: Guang YANG ¹ ; Hai-zhen LIANG ² ; Jie ZHANG ² ; Xiao-juan CHEN ² ; Bao-lin GUO ³ ; Bai-ping MA ¹
Author Information

1. Guangdong Pharmaceutical University, Guangzhou 510006, China; Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Publication Type:Research Article
Keywords: Cucurbitaceae; italic>Gynostemma pentaphyllum; triterpenoid saponin; isolation; structural elucidation
From: Acta Pharmaceutica Sinica 2024;59(8):2288-2294
CountryChina
Language:Chinese
Abstract: Damarane-type triterpene saponins are the main active ingredients in Gynostemma pentaphyllum (Thunb.) Makino. By using D101 macroporous adsorption resin and silica gel open-columns, C18 medium-pressure column chromatography, and preparative high performance liquid chromatography, we isolated four compounds from the leaves of G. pentaphyllum planted in Guangxi Zhuang Autonomous Region. Their structures were determined by comprehensive analyses of MS, NMR data and circular dichroism spectroscopy and identified as (3β,12β)-dihydroxy-25-peroxyhydrohydrodammarane-20,23-diene-3-O-[β-D-glucopyranosyl(1→2)]-β-D-glucopyranoside (1), (3β,12β,24S)-trihydroxydammarane-20,25-diene-3-O-[β-D-glucopyranosyl(1→2)]-β-D-glucopyranoside (2), (3β,20α)-dihydroxy-24-en-12β,22S-epoxydammarane-3-O-[β-D-glucopyranosyl(1→2)]-β-D-glucopyranoside (3), (3β,12β,20S)-trihydroxydammarane-24-en-3-O-[6-O-acetyl-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-β-D-xylopyranosyl(1→3)-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside (4). Compounds 1-4 are new dammarane-type triterpene saponins.