Identification of Zg02 metabolites in rats by UPLC-Q-TOF/MSE
10.16438/j.0513-4870.2024-0060
- VernacularTitle:UPLC-Q-TOF/MSE技术分析鉴定Zg02在大鼠体内的代谢产物
- Author:
Man ZHANG
1
;
Rui CHEN
2
;
Ke-rong HU
1
;
Yao CHENG
1
;
Jing HUANG
3
Author Information
1. Guizhou Medical University, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R & D, Guiyang 550004, China; School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
2. Guizhou Medical University, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R & D, Guiyang 550004, China
3. School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Research Article
- Keywords:
UPLC-Q-TOF/MSE;
metabolite;
rats in vivo
- From:
Acta Pharmaceutica Sinica
2024;59(8):2305-2312
- CountryChina
- Language:Chinese
-
Abstract:
In this study, plasma, urine and fecal samples were collected from rats after intragastric administration of novel insulin sensitizer Zg02 (20 mg·kg-1). The ultra-performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry (UPLC-Q-TOF/MSE) techniques was used to obtain the molecular ion and mass spectrometry fragment ion information of the compound, and the metabolites were quickly analyzed by combining with UNIFI metabolite software. The results showed that a total of 12 metabolites were inferred in rats after a single gavage of Zg02 (20 mg·kg-1), including 5, 7 and 11 metabolites in plasma, urine and feces (including cross-analysis), and the metabolic pathways were mainly glucuronidation and glucosylation. All animal protocols were approved by the Animal Ethics Committee of Guizhou Medical University (No. 2100856).
