The material basis and mechanism of action of anti-inflammatory effects of simplified Zhiqin Decoction
10.16438/j.0513-4870.2023-0817
- VernacularTitle:简化栀芩汤的抗炎药效物质基础及作用机制研究
- Author:
Kun WANG
1
;
Yang LIU
1
;
Yue YIN
1
;
Xiao XIAO
1
;
Xue-jiao ZHOU
1
;
Zhi-ying YUAN
1
;
Liang-hong YE
2
;
Xiao-yu XU
3
Author Information
1. School of Pharmacy & School of Traditional Chinese Medicine, Southwest University, Chongqing 400715, China
2. Tongliang District Hospital of Traditional Chinese Medicine in Chongqing, Chongqing 402560, China
3. School of Pharmacy & School of Traditional Chinese Medicine, Southwest University, Chongqing 400715, China; Southwest University Hospital, Chongqing 400715, China; State Administration of Traditional Chinese Medicine High-level Chinese Medicine Pharmacology Key Construction Discipline, Chongqing 400715, China; Chongqing Chinese Medicine Rehabilitation Key Construction Discipline, Chongqing 400715, China
- Publication Type:Research Article
- Keywords:
simplified Zhiqin Decoction;
inflammatory pharmacodynamics;
mechanism of action;
pharmacodynamic material basis;
network pharmacology
- From:
Acta Pharmaceutica Sinica
2024;59(8):2245-2254
- CountryChina
- Language:Chinese
-
Abstract:
The anti-inflammatory effect of simplified Zhiqin Decoction was observed by using lipopolysaccharide (LPS)-induced inflammation mouse model. The main chemical constituents and the main mechanism of action of simplified Zhiqin Decoction were predicted by network pharmacology. Animal experiments verified the anti-inflammatory mechanism of simplified Zhiqin Decoction (this experiment was approved by the Animal Experiment Ethics Committee of Southwest University, approval number: IACUC-20210825-02). Simplifying Zhiqin Decoction has a significant anti-inflammatory effect on inflammatory mice, can significantly improve the overall macro shape of mice, reduce body temperature, water intake, increase the number of autonomous activities; alleviate liver, lung, spleen, thymus inflammation and pathological damage; decrease tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6, nitric oxide (NO), prostaglandin E2 (PGE2) content in serum and urine. The contents of serum immune factors IgG, IgA and IgM were increased. Network pharmacology predicted 66 potential anti-inflammatory active components of simplified Zhiqin Decoction involving 132 inflammatory targets, and the key anti-inflammatory signaling pathway involved PI3K/AKT, TNF, JAK-STAT, etc. Animal experiments show that simplified Zhiqin Decoction can significantly reduce the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway related proteins in lung tissue of inflammatory mice. The results of this study showed that simplified Zhiqin Decoction had significant anti-inflammatory effects, and its main anti-inflammatory components were quercetin, β-sitosterol, kaempferol, wogonin, stigmasterol, luteolin, neobaicalein, etc. The main mechanism of its anti-inflammatory action is that it significantly inhibits the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway protein.
