Preliminary study of the transport and absorption mechanism of Astragalus polysaccharide-Ⅱ on M cells
10.16438/j.0513-4870.2024-0103
- VernacularTitle:黄芪多糖APS-Ⅱ在M细胞上的转运吸收机制初探
- Author:
Wan-wan LÜ
1
;
Ke LI
2
;
Shi-hong FENG
1
;
Yu-wei WEN
1
;
Xue-mei QIN
1
;
Yu-guang DU
3
;
Zhen-yu LI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education of Shanxi University, Taiyuan 030006, China; Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China
2. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education of Shanxi University, Taiyuan 030006, China; Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China; Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
- Publication Type:Research Article
- Keywords:
italic>Astragalus polysaccharide-Ⅱ;
M cell;
transport inhibitor;
mechanism of absorption
- From:
Acta Pharmaceutica Sinica
2024;59(10):2820-2827
- CountryChina
- Language:Chinese
-
Abstract:
To explore the absorption mechanism of APS-Ⅱ in vivo by establishing M cell model. First, Astragalus polysaccharides (APS) was divided into two different molecular weight polysaccharides APS-I ( > 2 000 kDa) and APS-II (10 kDa) by ultrafiltration, and APS-II (10 kDa) was prepared and fluorescently labeled. Meanwhile, M cell model was constructed by Caco-2 cells and Raji cells. The M cell model was treated with transport inhibitors to explore the transport of APS-Ⅱ on M cells. The results show that FITC has been successfully labeled to the end of APS-Ⅱ, and the M cell model was successfully constructed, which found that APS-Ⅱ could be transported by M cells, and four transport inhibitors of 5-(N-ethyl-N-isopropyl) amiloride (EIPA), genistein, dynasore and nocodazole indicated that APS-II may enter cells through clathrin and caveolin-mediated endocytosis.
