Urine metabolomics study of Psoralea corylifolia in improving learning and memory ability in APP/PS1 mice
10.16438/j.0513-4870.2023-0804
- VernacularTitle:补骨脂改善APP/PS1小鼠学习记忆能力的尿液代谢组学研究
- Author:
Yue QIAO
1
;
Ao XUE
1
;
Yue ZHANG
1
;
Hong-dan XU
2
;
Guang LI
3
;
Ji-hui ZHAO
4
;
Jing HU
2
;
Ning ZHANG
1
Author Information
1. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. Department of Pharmacy, Wuxi Higher Health Vocational Technology School, Wuxi 214028, China
3. Institute of Medicinal Plant Development Yunnan Branch, Chinese Academy of Medical Sciences, Jinghong 666100, China
4. College of Pharmacy, Hunan University of Medicine, Huaihua 418000, China
- Publication Type:Research Article
- Keywords:
italic>Psoralea corylifolia Linn.;
Alzheimer's disease;
metabolomics;
energy metabolism;
APP/PS1 mice
- From:
Acta Pharmaceutica Sinica
2024;59(4):1010-1016
- CountryChina
- Language:Chinese
-
Abstract:
Urine nontargeted metabolomics technology was developed for investigating the effect and mechanism of improving learning and memory ability in APP/PS1 mice of Psoralea corylifolia. All animal experiments were approved by the Animal Ethics Committee of Heilongjiang University of Chinese Medicine (Approval No.: 2020092502). Sixteen APP/PS1 mice were randomly divided into the model group and Psoralea corylifolia group (0.5 g·kg-1), and eight male C57BL/6J mice of the same background were selected as control group, step-through test and novel object recognition were used as evaluation indexes. Changes in urine endogenous metabolites of mice from eachgroup were determined by ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS), and differential metabolites were screened, and metabolic pathway enrichment analysis was performed. The results of pharmacodynamic investigation showed that Psoralea corylifolia can reduce the dark incubation period and number of errors in APP/PS1 mice (P < 0.01) and improve the new object recognition index of APP/PS1 mice (P < 0.01). Metabolomics analysis identified 15 differential metabolites, and 9 differential metabolites were significantly call back by Psoralea corylifolia. Metabolic pathway analysis showed that histidine metabolism, citric acid cycle, taurine and hypotaurine metabolism and glucose metabolism were the main metabolic pathways of Psoralea corylifolia in improving learning and memory ability. These studies suggest that Psoralea corylifolia improves the learning and memory ability of APP/PS1 mice, and its mechanism may be related to improving mitochondrial dysfunction, reducing peripheral histamine level, regulating energy metabolism disorders and antioxidant levels.
