Mechanism of placental polypeptide in improving ovarian function and oxidative stress in rats with premature ovarian failure
- VernacularTitle:胎盘多肽改善卵巢早衰大鼠卵巢功能及氧化应激的作用机制
- Author:
Juan JIN
1
;
Li XIAO
2
;
Qian PU
3
;
Hua ZHANG
4
;
Lei YU
2
Author Information
1. School of Clinical Medicine，Guizhou Medical University，Guiyang 550004，China;Dept. of Obstetrics，Qiannan Prefecture People’s Hospital，Guizhou Duyun 558099，China
2. School of Clinical Medicine，Guizhou Medical University，Guiyang 550004，China
3. Central Laboratory of Guiyang Maternal and Child Health Care Hospital，Guiyang 550004，China
4. Dept. of Obstetrics，Qiannan Prefecture People’s Hospital，Guizhou Duyun 558099，China
- Publication Type:Journal Article
- Keywords:
placental polypeptide;
premature ovarian failure;
Runt-related transcription factor 3;
Notch;
ovarian function
- From:
China Pharmacy
2024;35(21):2609-2615
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the impact of placental polypeptide on the Runt-related transcription factor 3 （RUNX3）/ Notch signaling pathway in rats with cyclophosphamide-induced premature ovarian failure （POF）， and its effects on ovarian function and oxidative stress. METHODS A POF rat model was induced by intraperitoneal injection of cyclophosphamide. Sixty POF rats of the model were randomly assigned to model group， low-dose （1 mg/kg） and high-dose （2 mg/kg） placental polypeptide groups， placental polypeptide plus empty vector group [placental polypeptide （2 mg/kg）， empty vector （1 μg）]， and placental polypeptide plus RUNX3 silencing group [placental polypeptide （2 mg/kg）， RUNX3 small interfering RNA （1 μg）]， with 12 rats in each group. Additionally， 12 healthy rats were selected as a control group. The intervention lasted for 4 weeks for all groups. After the final administration， the levels of sex hormones [estradiol （E2）， anti-Müllerian hormone （AMH）， follicle- stimulating hormone （FSH）] in rat serum and oxidative stress indicators [malondialdehyde （MDA）， superoxide dismutase （SOD）， catalase （CAT）， reactive oxygen species （ROS）] in ovarian tissue were measured. The pathology of rat ovarian tissue and apoptosis of ovarian granulosa cells were examined； the expression of apoptosis-related proteins [caspase-3， cleaved-caspase-3， B-cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）and RUNX3/Notch signaling pathway-related proteins in rat ovarian tissue were detected. RESULTS Compared with the model group， the low and high doses of placental polypeptide groups showed an increase in the number of normal follicles and a marked decrease in the number of atretic follicles， with significant increases in the serum levels of E2 and AMH， the ovarian tissue levels of SOD and CAT， as well as the expression levels of Bcl-2， RUNX3 and Notch1 proteins， and significant decreases in the serum FSH levels， the ovarian granulosa cell apoptosis rate， and the ovarian tissue levels of MDA and ROS， as well as the expression levels of cleaved-caspase-3， caspase-3 and Bax proteins （P＜0.05）； the changes in the high-dose group were significantly more pronounced （P＜0.05）. Compared with the high-dose placental polypeptide group and the placental polypeptide plus empty vector group， the placental polypeptide plus RUNX3 silencing group showed a decrease in the number of normal follicles and a significant reversal of the above indicators （P＜0.05）. CONCLUSIONS Placental polypeptide may alleviate the symptoms of rats with POF by upregulating the RUNX3/Notch signaling pathway， improving sex hormone secretion， oxidative stress， ovarian granulosa cell apoptosis and ovarian function.
