Effect and mechanism of casticin on wound healing in rats with superficial second-degree burn
- VernacularTitle:紫花牡荆素对浅Ⅱ度烧伤大鼠创面愈合的影响及机制研究
- Author:
Guohui ZHANG
1
;
Haibo ZHANG
2
;
Dongsheng CAO
3
Author Information
1. Dept. of Plastic and Wound Repair Surgery，the Second Hospital of Anhui Medical University，Hefei 230601，China;The Second School of Clinical Medicine，Anhui Medical University，Hefei 230601，China;Dept. of Cosmetic Medicine，Binzhou Medical University Hospital，Shandong Binzhou 256603，China
2. Dept. of Cosmetic Medicine，Binzhou Medical University Hospital，Shandong Binzhou 256603，China
3. Dept. of Plastic and Wound Repair Surgery，the Second Hospital of Anhui Medical University，Hefei 230601，China
- Publication Type:Journal Article
- Keywords:
casticin;
superficial second-degree burn;
wound healing;
PI3K/AKT/mTOR signaling pathway
- From:
China Pharmacy
2024;35(21):2603-2608
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the mechanism by which casticin （CAS） promotes wound healing in superficial second- degree burned rats through phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT）/mammalian target of rapamycin （mTOR） signaling pathway. METHODS Rats were randomly assigned to a control group （physiological saline）， a model group （5% sodium carboxymethyl cellulose blank matrix）， low- and high-dose CAS groups （15 mL of CAS solution at concentrations of 30 and 60 mmol/L， respectively）， a high dose CAS plus LY294002 group [a mixture of 15 mL CAS solution （60 mmol/L） and PI3K inhibitor LY294002 solution （20 μmmol/L）]， and a positive control group （thick application of Jingwanhong ointment 0.5-1.0 cm）， with 15 rats in each group. Except for the control group， the other groups were subjected to a superficial second-degree burn rat model by igniting a mixture of burn fuel on the skin surface， and the administration was applied topically within two hours after the burn. After 28 days of administration， the wound healing rate of the burned rats was calculated， the histopathological changes in the central tissue of the rat’s burned wound were observed， and the levels of tumor necrosis factor α （TNF-α）， interleukin-1β （IL- 1β）， IL-6， matrix metalloproteinase-2 （MMP-2）， MMP-9 and vascular endothelial growth factor （VEGF） in the serum of rat were detected. The phosphorylation levels of related proteins in the PI3K/AKT/mTOR signaling pathway in the burned tissue of the rats were also detected. RESULTS Compared with the control group， the rats in the model group showed epidermal and superficial dermal damage in skin tissue， poor healing status， inflammatory cell infiltration， and incomplete tissue structure. The levels of IL-6， IL-1β， TNF-α， MMP-2 and MMP-9 in the serum were significantly increased （P＜0.05）， and the levels of VEGF in the serum and the phosphorylation levels of E-mail：caodongsheng@ahmu.edu.cn PI3K， AKT， mTOR proteins in the central tissue of the burned wound were significantly decreased （P＜0.05）. Compared with the model group， the pathological changes and the levels of the above indicators in the serum and central tissue of the burned wound in the CAS low- and high-dose groups were significantly reversed （P＜0.05）， and the changes in the CAS high-dose group were significantly more pronounced than those in the CAS low- dose group （P＜0.05）. The addition of the PI3K inhibitor LY294002 could reverse the promoting effect of CAS on the wound healing of superficial second-degree burned rats （P＜0.05）. CONCLUSIONS CAS can promote the healing of superficial second- degree burned wounds in rats， and its mechanism of action may be related to the activation of the PI3K/AKT/mTOR signaling pathway.
