Chemical synthesis and antibody affinity of epitope fragments from Helicobacter pylori lipopolysaccharide
10.11665/j.issn.1000-5048.2024031201
- VernacularTitle:幽门螺杆菌脂多糖抗原表位片段的化学合成及抗体亲和力研究
- Author:
Hui ZHAO
1
;
Xiaopeng ZOU
;
Lei XIAO
;
Jing HU
;
Jian YIN
Author Information
1. 江南大学生物工程学院糖化学与生物技术教育部重点实验室,无锡 214122
- Keywords:
Helicobacter pylori;
lipopolysaccharide;
α-1,6-glucan;
glycan microarray;
antibody affinity
- From:
Journal of China Pharmaceutical University
2024;55(5):645-656
- CountryChina
- Language:Chinese
-
Abstract:
Helicobacter pylori(Hp)is responsible for chronic gastritis,peptic ulcers,and even gastric cancers.Currently,there is no vaccine to prevent or treat Hp infections.Here,we described the chemical synthesis of α-1,6-glucans with different lengths(di-to hexasaccharide),which are present in the core oligosaccharide of Hp lipopolysaccharide(LPS).The 1,2-cis-glucosidic bonds were constructed successfully using a synergistic glycosylation strategy based on acyl remote participation and solvent effects.The results of glycan microarrays indicated that all synthesized α-1,6-glucan fragments possessed a strong binding to IgG antibodies in both rabbit serum immunized with Hp O1 LPS and patient serum infected with Hp.The α-1,6-linked trisaccharide exhibited strong binding affinity to anti-LPS rabbit IgG antibodies.The α-1,6-glucan trisaccharide and pentasaccharide elicited a strong response to IgG antibodies in sera of most Hp-infected patients.Some patients'sera exhibited strong binding activity with α-1,6-linked disaccharide.The results suggest that the α-1,6-glucan disaccharide,trisaccharide and pentasaccharide could be important carbohydrate antigen fragments in Hp lipopolysaccharide.
