Engineering The Neck Hinge Reshapes The Processive Movement of Kinesin-3
10.16476/j.pibb.2024.0269
- VernacularTitle:改造颈部铰链区重塑驱动蛋白3的持续运动能力
- Author:
Dong LI
1
;
Jin-Qi REN
1
;
Yin-Long SONG
2
;
Xin LIANG
2
;
Wei FENG
1
Author Information
1. Key Laboratory of Biomacromolecules (CAS), National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2. School of Life Sciences, Tsinghua University, Beijing 100084, China
- Publication Type:Journal Article
- Keywords:
intracellular transport;
molecular motor;
kinesin-3;
neck hinge;
processive movement
- From:
Progress in Biochemistry and Biophysics
2024;51(10):2730-2740
- CountryChina
- Language:English
-
Abstract:
ObjectiveIn kinesin-3, the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A. The function of this neck hinge for controlling processive movement, however, remains unclear. MethodsWe made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay. ResultsIn KIF13B, the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity, while the removal of this proline increases the both. In KIF1A, the deletion of entire flexible neck hinge merely enhances the processivity. The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1. ConclusionThe neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility, which provides a novel strategy to reshape the processive movement of kinesin motors.
