Advances of Volume Electron Microscopy
10.16476/j.pibb.2024.0324
- VernacularTitle:体电子显微学前沿
- Author:
Yan ZHANG
1
;
Xi CHEN
2
;
Xi-Xia LI
3
;
Lin-Lin LI
2
;
Lian-Wan CHEN
1
;
Hua HAN
2
;
Fei SUN
1
Author Information
1. Key Laboratory of Biological Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2. Key Laboratory of Brain Cognition and Brain-inspired Intelligence Technology, Micro Reconstruction & Intelligent Analysis Team, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
3. Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Publication Type:Journal Article
- Keywords:
volume electron microscopy;
scanning electron microscopy;
transmission electron microscopy;
sample preparation;
image processing;
cross-scale;
deep learning;
ultrastructure
- From:
Progress in Biochemistry and Biophysics
2024;51(10):2430-2455
- CountryChina
- Language:Chinese
-
Abstract:
Volume electron microscopy (vEM) imaging technology was rapidly developed in recent years. It has been the advanced technology to solve high-resolution three-dimensional structures of biological samples. Much wonderful work has revealed the fine structure and interactions of intracellular organelles, the ultrastructure of tissues, and even the three-dimensional structure of entire small biological organisms. With the continuous improvement of resolution, scale and throughput, vEM is becoming more and more widely used in medicine, life sciences, clinical diagnostics and other fields. As a result, this technology has been rated by Nature as one of the seven most noteworthy frontier technologies to watch in 2023. However, the development and application of vEM-related technologies started late in China and need to be further promoted. We write this review to introduce all related vEM technologies, covering the development history of vEM, technology classification, sample preparation, data collection, image processing, etc., which is convenient for people in various fields to understand, learn, apply and further develop this technology.
