Comparative study on primary culture of four strains of mouse keratinocytes
10.19405/j.cnki.issn1000-1492.2023.06.019
- Author:
Yanling Guan
1
;
Pingping Zhang
1
;
Xiu Chen
1
;
Liping Jiang
1
;
Wei Wei
1
;
Yang Ma
1
Author Information
1. Institute of Clinical Pharmacology，Anhui Medical University，Key Laboratory of Anti-inflammatory and Immune Medicine，Ministry of Education，Anhui Collaborative Innovation Center for Anti-inflammatory and Immune Drugs，Rheumatoid Arthritis Research Center，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
keratinocytes;
primary culture;
mouse epidermis;
proliferation;
differentiation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):989-995
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To isolate，culture and identify adult mouse dorsal keratinocyte ( KC) in vitro and to com- pare of the proliferation and differentiation capabilities of four adult mouse dorsal skin KC．
Methods :Four kinds of adult mouse dorsal skin were taken，the subcutaneous adipose tissue was peeled，and KC was obtained by 0. 25% trypsin digestion ; KC were identified using immunofluorescence method，cell growth status was observed under microscopy ，different seed plate times and densities were designed ，and cell proliferation viability was detected by CCK-8，EdU and high connotation method．Four adult mouse KC differentiation was detected by Western blot.
Results : Trypsin digestion was used to extract four strains of adult mouse back skin KC，and the Kunming ( KM) mice had the lowest number of KC and C57BL /6 to extract the highest number of cells for the same area of skin tis- sue．BALB / c，KM，and nude mouse had higher plate-laying efficiency at a density of 8 × 103 /well，respectively， and C57BL /6 had higher plate-laying efficiency at a density of 1. 6 × 104 /well．Among the four strains of mice，the primary KC of BALB / c mice was weaker than that of C57BL /6 mice，and there was no statistical difference in the degree of differentiation．
Conclusion : The proliferation and differentiation capacity of four adult mouse primary KC on the dorsal skin is successfully isolated and compared by a simple and easy method，which provided a certain ex- perimental basis for the strain selection of mice with skin-related diseases.
- Full text:20241014173453599484种品系小鼠角质形成细胞原代培养比较研究_关艳玲.pdf
