The experimental study on the construction of osteoblast specific recognition polypeptide sustained⁃release system on titanium implant surface
10.19405/j.cnki.issn1000-1492.2023.07.019
- Author:
Gang Wang
1
;
Si Li
1
;
Yufeng Wu
2
;
Mingyue Wu
3
Author Information
1. Stomatological College of Anhui Medical University, Hefei 230032
2. Dept of Stomatology, Hangzhou Traditional Chinese Medicine Hospital , Hangzhou 310005
3. Dept of Government Afairs District Outpatient , The Afiliated Stomatological Hospital of Anhui Medical University, Hefei 231699 ; Anhui Oral Disease Research Center Laboratory, Hefei 230032
- Publication Type:Journal Article
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1178-1183
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To construct the sodium alginate⁃chitosan polyelectrolyte multilayer membrane structure on the surface of alkaline heat treated titanium by LBL technology , and to combine the peptide that specifically bind to
osteoblasts with this structure to explore their slow⁃release effects.
Methods :After the smooth titanium surface was heat treated with NaOH , sodium alginate and chitosan were alternately adsorbed and deposited on the surface by layer by layer self⁃assembly method to form a polyelectrolyte multilayer film structure , and the multilayer film was used to load the peptide that specifically bind to osteoblasts , construct slow⁃release system , and evaluate the slowrelease effect.
Results :The sample surface was tested by contact angle measuring instrument , field emission scanning electron microscope , fluorescence microscope , X ⁃ray photoelectron spectrometer, Fourier transform infrared
spectroscopy and ultraviolet spectrophotometer to confirm that the sodium alginate⁃chitosan multilayer film structure was successfully constructed on the surface of titanium sheets , and the peptide that specifically bind to osteoblasts were successfully loaded.
Conclusion :The surface of titanium implants is self⁃assembled layer by layer to form a multilayer membrane structure and successfully loaded with the peptide that specifically bind to osteoblasts , and the sustained release system of the peptide is constructed , which has a remarkable sustained release effect and is expected to promote osseointegration on the surface of titanium implants.
- Full text:2024101016523874762钛种植体表面构建成骨细胞特...识别多肽缓释系统的实验研究_王刚.pdf
