Therapeutic effect of deferone on iron metabolism disorders in liver inj ury of chronic infected Toxoplasma gondii
10.19405/j.cnki.issn1000-1492.2023.07.006
- Author:
Min Liu
1
;
Nannan Gao
1
;
Chong Wang
1
;
Yihong Cai
1
Author Information
1. Dept of Health Inspection and Quarantine , Anhui Medical University, Hefei 230032; Dept ofPathogen Biology and Provincial Laboratories of Pathogen Biology and Zoonoses ,Hefei 230032; Anhui Provincial Laboratory of Zoonoses ofHigh Institutions ,Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
deferione;
iron metabolism;
liver damage;
ferritin;
Toxoplasma infection
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1094-1098
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the changes of iron and other metal elements and inflammatory factor γ interferon (IFN⁃γ) in the liver after taking deferiprone (DFP) in mice with chronic infection of Toxoplasma gondii , and to investigate the efficacy of DFP in the treatment of liver injury caused by chronic infection of Toxoplasma gondii.
Methods :6 ⁃week⁃old female C57BL/6 mice were randomly divided into four groups : control group , control + DFP group , TgCtWh6 group , TgCtWh6 + DFP group , with 6 mice in each group , and sterile gavage PBS in control group. Daily gavage was 50 μg/kg DFP in TgCtWh6 + DFP group , 20 toxoplasma cysts in TgCtWh6 group , 20 toxoplasmosis cysts in TgCtWh6 + DFP group , 20 toxoplasmosis cysts with daily gavage of 50 μg/kg DFP , and mice were sacrificed and liver tissues were taken after 45 days. Inductively coupled plasma mass spectrometry (ICP⁃MS) was used to detect the content of iron and other metal elements in mouse liver tissues. Real⁃time PCR (qRT⁃PCR) and Western blot were used to detect the mRNA levels and protein levels of IFN⁃γ and ferritin heavy chain (Fth) in mouse liver tissues , respectively.
Results :Compared with the TgCtWh6 group , the Fth content in the liver (P < 0. 01) was significantly reduced in the liver of the TgCtWh6 + DFP group after taking DFP , the iron metabolism disorder was effectively improved , the IFN⁃γ content in the liver was significantly lowered (P < 0. 01) , and the inflammatory response of the liver was also reduced.
Conclusion :DFP can effectively improve iron metabolism disorders in the liver after infection with Toxoplasma gondii and reduce inflammatory damage in the liver.
- Full text:2024100916565134386去铁酮对弓形虫慢性感染小鼠...损伤中铁代谢紊乱的治疗作用_刘敏.pdf
