Effect of Bushen Huoxue Prescription on Ventricular Remodeling and Expression of AVP and AQPs in Heart and Brain of Rat Model of Heart Failure after Myocardial Infarction
10.13422/j.cnki.syfjx.20241424
- VernacularTitle:补肾活血方对心梗后心衰大鼠心室重构及心脑组织AVP、AQPs表达的影响
- Author:
Xiaoteng HE
1
;
Rui XU
1
;
Yan ZHANG
2
Author Information
1. Liaoning University of Traditional Chinese Medicine （TCM）， Shenyang 110847， China
2. The Affiliated Hospital of Liaoning University of TCM， Shenyang 110032， China
- Publication Type:Journal Article
- Keywords:
heart failure;
Bushen Huoxue prescription;
aquaporins;
cardiac function;
ventricular remodeling
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(21):130-137
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of Bushen Huoxue prescription on the expression of arginine vasopressin （AVP） and aquaporins （AQP）1 and AQP4 in the brain and AQP1， AQP4， and AQP7 in the myocardial tissue of the rat model of heart failure after myocardial infarction. MethodSixty healthy male SD rats were selected and randomized into sham surgery， model， Bushen Huoxue prescription， and tolvaptan groups. The rats in the sham surgery group only had their coronary arteries exposed without ligation， while those in the other groups were subjected to ligation of the left coronary artery combined with exhaustive swimming and starvation for the modeling of heart failure after myocardial infarction. After the successful modeling， the rats in the tolvaptan （1.35 mg·kg-1） and Bushen Huoxue prescription （15.75 g·kg-1） groups were treated with corresponding drugs， and those in the sham surgery and model groups were treated with an equal volume of normal saline for 4 weeks. Color doppler echocardiography was employed to measure changes of the left ventricular structure and function in rats. Enzyme-linked immunosorbent assay （ELISA） was employed to measure the serum level of N-terminal pro-B-type natriuretic peptide （NT-proBNP）. Hematoxylin-eosin （HE） staining was conducted to reveal the pathological changes in the myocardial tissue. Western blotting was employed to measure the protein levels of AVP， AQP1， and AQP4 in the brain tissue and AQP1， AQP4， and AQP7 in the myocardial tissue. Real-time fluorescence quantitative PCR was conducted to determine the mRNA levels of AVP， AQP1， and AQP4 in the brain tissue and AQP1， AQP4， and AQP7 in the myocardial tissue. ResultCompared with the sham surgery group， the model group showed increased left ventricular internal diameter （LVID） （P<0.01）， decreased ejection fraction （EF） and short axis fractional shortening rate （FS） （P<0.01）， increased left ventricular septal and posterior wall thicknesses （LVS and LVPW） （P<0.01）， elevated serum NT-proBNP level （P<0.01）， edema and vacuolar degeneration of nerve cells in the brain tissue， necrosis of myocardial cells and rupture of myocardial fibers in the infarcted area， and upregulated mRNA and protein levels of AVP， AQP1， and AQP4 in the brain tissue （P<0.01） and AQP1， AQP4， and AQP7 in the myocardial tissue （P<0.01）. Compared with the model group， tolvaptan and Bushen Huoxue prescription restored LVID， EF， FS， LVS， and LVPW （P<0.05， P<0.01）， lowered the serum NT-proBNP level （P<0.05）， alleviated the edema of nerve cells in the brain tissue， improved the arrangement of myocardial fibers， mitigated the necrosis of myocardial cells， and downregulated the mRNA and protein levels of AVP， AQP1， and AQP4 in the brain tissue （P<0.01） and AQP1， AQP4， and AQP7 in the myocardial tissue （P<0.05， P<0.01）. ConclusionBushen Huoxue prescription can regulate cardiovascular and cerebrovascular fluid metabolism disorders， delay ventricular remodeling， and improve the cardiac function by downregulating the expression of AVP， AQP1， and AQP4 in the brain tissue and AQP1， AQP4， and AQP7 in the myocardial tissue in the rat model of heart failure after myocardial infarction.
