Clinical outcomes of preimplantation genetic testing of vitrification⁃thawing blastocysts
10.19405/j.cnki.issn1000-1492.2023.08.022
- Author:
Dan Kuang
1
;
Yan Hao
1
;
Dawei Chen
2
;
Zhiguo Zhang
2
;
Qing Zhang
2
;
Yiqi Yin
2
;
Ning Wang
2
;
Ping Zhou
2
;
Zhaolian Wei
2
;
Yunxia Cao
2
Author Information
1. Dept of Obstetrics and Gynecology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022 ; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University) , Hefei 230032; Key Laboratory ofPopulation Health Across Life Cycle (Anhui Medical University) , Ministry of Education of the People ′s Republic of China , Hefei 230032
2. Dept of Obstetrics and Gynecology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022 ; Anhui Province Key Laboratory ofReproductive Health and Genetics , Hefei 230032; Anhui Provincial Engineering Research Center of Biopreservation and Artificial Organs , Hefei 230032; Anhui Provincial Institute of Translational Medicine , Hefei 230032
- Publication Type:Journal Article
- Keywords:
blastocyst;
biopsy;
vitrification;
thawing;
preimplantation genetic testing
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1380-1386
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To analyze the data related to the clinical outcome of preimplantation genetic testing (PGT)
for double frozen , double biopsied blastocysts and double frozen , once biopsied blastocysts , in order to expand the existing data and provide some guidance for the clinical value and safety of PGT for frozen⁃thawed embryos .
Methods :Retrospective analysis was made on the 38 PGT cycles of frozen⁃thawed blastocysts . According to the frequency of biopsy , cases in the study were divided into two groups : double frozen , double biopsy ( DFDB) group and double frozen , single biopsy ( DFSB) group . The freezing method was vitrification .
Results : There were 24 patients in DFDB group , 34 blastocysts were not diagnosed in the last PGT cycle , 32 blastocysts survived after thawing , and the survival rate of thawed blastocysts was 94. 12% . After the second biopsy of these 32 blastocysts , genetic testing was performed , and all of them were definitely diagnosed , including 15 normal blastocysts (46. 88% ) and 17 abnormal blastocysts (53 . 13% ) . There were 14 patients in DFSB . The remaining 50 blastocysts in the last ICSI cycle were thawed and all blastocysts survived after thawing . Biopsy of these 50 blastocysts and genetic analysis showed that 47 blastocysts were diagnosed , including 9 normal blastocysts (18 . 00% ) , 28 abnormal blastocysts (56. 00% ) , 10 mosaic blastocysts (20. 00% ) , and 3 undiagnosed blastocysts (6. 00% ) . In DFDB group and DFSB group , 8 patients and 5 patients transferred the normal blastocystswhich all survivedafter thawing . There were 5 clinical pregnancies and 3 clinical pregnancies , respectively . One healthy live birth was obtained respectively in each group .
Conclusion : Acceptable pregnancy rate can be obtained whatever DFSB or DFDB blastocyst , which is
of clinical value . However , due to the small sample size , we need to expand the sample size to further explore its
safety .
- Full text:2024081910041418096解冻复苏囊胚行植入前遗传学检测临床结局分析_匡丹.pdf
