Expression and functional mechanism of KCTD7 gene in hepatocellular carcinoma
10.19405/j.cnki.issn1000-1492.2023.08.018
- Author:
Ya Zhao
1
;
Nan Zhao
1
;
Wei Zhao
1
;
Wenying Yu
1
;
Xiao Fang
1
Author Information
1. Institute of Translational Medicine , Medical College , Yangzhou University , Jiangsu Key Laboratory of Experimental & Translational Non⁃coding RNA Research , Yangzhou University , Yangzhou 225009
- Publication Type:Journal Article
- Keywords:
Toll⁃like receptor 7;
NF⁃κB;
trigeminal neuralgia;
inflammatory factors
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1354-1360
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Using RNA sequencing data from The Cancer Genome Atlas (TCGA) to explore the expres⁃
sion , mechanism , and clinical significance of KCTD7 in hepatocellular carcinoma ( HCC) .
Methods : The RNA sequencing data and clinical information of HCC patients were downloaded from the TCGA database . Univariate and multivariate Cox regression analyses were used to explore the correlation between the expression level of the KCTD7 gene in HCC and the clinical information and prognosis of patients . Gene Set Enrichment Analysis ( GSEA) was used to predict possible pathways regulated by the KCTD7 gene in HCC . Single⁃sample GSEA (ssGSEA) was used to compare immune infiltration between KCTD7 high expression group and low expression group . KCTD7 knockdown HCC cell lines were established to explore its function and possible mechanism in HCC regulation .
Results:KCTD7 was highly expressed in HCC tissues compared to normal tissues (P < 0. 01) . The overall survival rate of HCC patients with high expression of KCTD7 gene was worse (P < 0. 05) . The expression level of KCTD7 was an independent risk factor affecting the overall survival of HCC patients . GSEA results showed that the KCTD7 gene was related to cell cycle signaling pathways . In the tumor microenvironment , high expression of the KCTD7 gene was positively correlated with activated CD4 + T cells , central memory CD4 + T cells , and natural killer cells . Knocking down KCTD7might inhibit HCC cell proliferation , impair cell cycle distribution , and promote apoptosis .
Conclusion : KCTD7 gene is highly expressed in HCC and affects the prognostic survival of HCC patients . Knocking down KCTD7 can inhibit the proliferation of HCC cells and promote apoptosis .
- Full text:2024081617062762782KCTD7基因在肝细胞癌中的表达和作用机制_赵雅.pdf
