Regulation effects and mechanism of liquiritin on immune function in gastric cancer-bearing mice
- VernacularTitle:甘草苷对胃癌荷瘤小鼠免疫功能的调节作用及机制研究
- Author:
Chunyan LAN
1
;
Xiaolan YANG
2
;
Xuefeng HE
1
;
Dan ZHAO
3
;
Haiyan YANG
1
Author Information
1. School of Pharmacy，Dazhou Vocational College of Chinese Medicine，Sichuan Dazhou 635000，China
2. Dept. of Traditional Chinese Medicine，School of Acupuncture and Moxibustion，Hospital of Chengdu University of Traditional Chinese Medicine，Chengdu 610000，China
3. Dept. of Clinical Pharmacology，Dazhou Hospital of Integrated Traditional Chinese and Western Medicine，Sichuan Dazhou 635000，China
- Publication Type:Journal Article
- Keywords:
liquiritin;
JAK2/STAT3 signaling pathway;
gastric cancer;
immune function
- From:
China Pharmacy
2024;35(15):1862-1867
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the regulation effects and mechanism of liquiritin （LIQ） on immune function in gastric cancer-bearing mice based on the Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） pathway. METHODS Gastric cancer cells MFC were injected subcutaneously to establish gastric cancer-bearing model of mice. The model mice were divided into model group， LIQ low-dose group （LIQ-L group， 20 mg/kg）， LIQ high-dose group （LIQ-H group， 40 mg/kg）， and high-dose LIQ+JAK2 activator coumermycin A1 group （LIQ-H+coumermycin A1 group， 40 mg/kg LIQ+1 mg/kg coumermycin A1）， with 12 mice in each group. Another 12 mice without modeling were set as normal group. Mice in each group were given the corresponding drug or normal saline by intragastric administration/intraperitoneal injection， once a day， for consecutive 14 days. After the last administration， the volume and mass of gastric cancer tumor and organ index were measured. The percentages of CD4+ and CD8+T lymphocytes were detected in peripheral blood. The histopathological morphology of gastric cancer tumor tissues was observed， and the expression levels of JAK2/STAT3 signaling pathway-related proteins and interleukin-6 （IL-6） protein in gastric cancer tumor tissues were detected. RESULTS Compared with the normal group， the thymus index， spleen index， and the percentage of CD8 T lymphocyte in the peripheral blood of mice were obviouslyincreased in model group （P＜0.05）， while the percentage of CD4+T lymphocyte in the peripheral blood were decreased （P＜0.05）. Compared with model group， the above indexes in LIQ-L group and LIQ-H group were significantly reversed （P＜0.05）， while the volume and mass of gastric cancer tumor， the phosphorylation levels of JAK2 and STAT3 protein and the expression level of IL-6 protein were significantly decreased in tumor tissue （P＜0.05）， and the effect of LIQ was in a dose-dependent manner （P＜0.05）； the tumor cells showed varying degrees of loose arrangement， vacuolization， and uneven distribution. JAK2 activator coumermycin A1 weakened the improvement effect of LIQ on the immune function of gastric cancer-bearing mice and its inhibitory effect on gastric cancer tumors （P＜0.05）. CONCLUSIONS LIQ can improve the immune function of gastric cancer-bearing mice by inhibiting the activation of JAK2/STAT3 signaling pathway， thus playing an anti-tumor role.
