Study on anti⁃hepatitis C virus NS3/4A protease activity of Lonicera fulvotomentosa based on fluorescence method and molecular docking
10.19405/j.cnki.issn1000-1492.2023.05.005
- Author:
Xin Yang
1
;
Junwei Xiao
2
;
Tingting Tang
2
;
Ying Wei
2
;
Ying Chen
2
;
Liangshan Xiang
2
;
Ying Zhou
2
Author Information
1. School ofBasic Medicine ,Guizhou University of Traditional Chinese Medicine ,Guiyang 550025
2. College ofPharmacy,Guizhou University of Traditional Chinese Medicine ,Guiyang 550025
- Publication Type:Journal Article
- Keywords:
Lonicera fulvotomentosa;
HCV NS3/4A protease;
activity determination;
molecular docking
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):731-734
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study anti⁃hepatitis C virus NS3/4A protease activity of Lonicera fulvotomentosa based on fluorescence method and molecular docking.
Methods:The activity of HCV NS3/4A protease inhibition of L.fulvotomentosa extracts was tested by fluorescence method. The binding of the main active ingredients to HCV NS3/4A virus protease was analyzed based on molecular docking.
Results:The water extract and alcohol extract of L.fulvotomentosa had a good inhibitory effect on the activity of HCV NS3/4A protease with IC50 of 0. 005 - 0. 019 mg/ml.Based on molecular coupling and interaction analysis , it was found that chlorogenic acid , isochlorogenic acid A , cynaroside and luteolin combined well with HCV NS3/4A protease , forming multiple hydrogen bonds.
Conclusion:The active components of L. fulvotomentosa against HCV NS3/4A protease activity were isochlorogenic acid A and luteolin.
- Full text:2024081022383991527基于荧光法及分子对接研究黄...病毒NS3_4A蛋白酶活性_杨欣.pdf
