Osteogenic potential of nerve growth factor incorporated gelatin methacryloyl beads: An in vitro study using MC3T3-E1
10.14815/kjdm.2024.51.2.109
- Author:
Min-Jeong LEE
1
;
Ji-Eun KIM
;
Jung-eun CHOI
;
Jae-Sung KWON
Author Information
1. Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea
- Publication Type:Original Article
- From:
Korean Journal of Dental Materials
2024;51(2):109-118
- CountryRepublic of Korea
- Language:English
-
Abstract:
Despite attempts to aid hard tissue regeneration using materials such as gelatin methacryloyl (GelMa), success has been limited. In this study, considering osteogenic differentiation and mineralization of MC3T3-E1 cells, nerve growth factor (NGF) was utilized for osteogenic differentiation by incorporating NGF into GelMA beads. GelMa beads with NGF (GBNGF) or without NGF (GB) were prepared. MC3T3-E1 cells were then encapsulated in GelMA beads fabricated by UV cross-linking. Live/Dead staining results showed that MC3T3-E1 cells in the GBNGF group exhibited more active cell-cell network formation and clearer spindle-shaped cell morphology than those in the GB group. Alizarin Red staining results revealed that MC3T3-E1 cells cultured in the GBNGF group had a higher amount of calcification indicated by a stronger red staining than those cultured in the GB group. Both results demonstrates that NGF could promote cell-cell and cell-matrix interactions, enhancing cell growth and osteogenic differentiation. In conclusion, the incorporation of NGF into GelMA beads can effectively promote growth and osteogenic differentiation of MC3T3-E1 cells. Therefore, GelMA beads containing NGF are expected to be utilized as a new therapeutic strategy for the regeneration of bone defects.
