The mitochondrial autophagy inhibitor Mdivi-1 improve the injury of epileptic hippocampal neurons by regulating mitochondrial unfolded protein response
10.19845/j.cnki.zfysjjbzz.2020.0525
- VernacularTitle:线粒体自噬抑制剂Mdivi-1调控线粒体未折叠蛋白反应改善癫痫海马神经元损伤
- Author:
Nanchang XIE
1
;
Liyuan DU
1
;
Yajun LIAN
1
Author Information
1. Department of Neurology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China
- Publication Type:Journal Article
- Keywords:
Mitochondrial unfolded protein response;
Mitophagy;
Epilepsy;
Mitochondrial division inhibitor 1
- From:
Journal of Apoplexy and Nervous Diseases
2020;37(12):1087-1090
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of mitochondrial unfolded protein response in epileptic hippocampal neurons induced by Mg2+-free solution and the effects of mitochondrial autophagy inhibitors Mdivi-1.Methods Epilepsy model was induced in primary cultured hippocampal neurons of SD rats in vitro by Mg2+-free solution,and further treated with mitochondrial autophagy inhibitor Mdivi-1.Mitochondrial ROS production were detected by MitoSox.The mitochondrial membrane potential was detected by Rhodamine123.The expression of C/EBP homologous protein CHOP,mitochondrial heat shock protein HSP60,mitochondrial protease ClpP,CytC release and caspase-3 activation were detected by Western blotting.Results (1)Compared with the control group,the expression of CHOP,HSP60 and ClpP in the AE group was significantly increased.Compared with the AE group,Mdivi-1 pretreatment enhanced the expression of CHOP,HSP60 and ClpP.(2)Compared with the control group,the production of ROS in the AE group increased,and the mitochondrial menmbrane potential decreased.Compared with the AE group,Mdivi-1 pretreatment significantly decreased the production of mitochondrial ROS and increased the mitochondrial membrane potential.(3)Compared with the control group,the release of CytC and the activation of caspase-3 in the AE group increased.Compared with the AE group,Mdivi-1 pretreatment decreased the release of CytC and caspase-3 activation.Conclusion Mitochondrial unfolded protein response is activated in epileptic hippocampal neurons,while inhibition of mitochondrial autophagy may play a protective role via enhancing mitochondrial unfolded protein response.
- Full text:2024073114515124420The mitochondrial autophagy inhibitor Mdivi-1 improve the injury of epileptic hippocampal neurons by regulating mitochondrial unfolded protein response.pdf
