Combined use of low oxygen and GDNF on induction of differentiation from neural stem cells into dopaminergic neurons in vitro
- VernacularTitle: 低氧联合GDNF诱导大鼠神经干细胞分化为TH阳性神经元的实验研究
- Author:
Juan DU
1
;
Changhong LIU
1
;
Hui LIANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Neural stem cells; Low oxygen; Dopaminergic neurons; Differentiation
- From: Journal of Apoplexy and Nervous Diseases 2021;38(4):322-325
- CountryChina
- Language:Chinese
- Abstract: Objective In this study,glial cell-derived neurotrophic factor (GDNF) were used to induce neural stem cells (NSCs) derived from brain tissue of rat embryo to directional differentiation at a low oxygen environment.Thereby,exploring the affects of low oxygen on differentiation of NSCs into dopaminergic neurons.In this condition.We got quite a lot of dopaminergic cells,which will lay the foundation for the next step of cell transplantation.Methods NSCs were isolated and cultured from cortex of 14~16 days Wistar rat embryo.Then,at a series of different oxygen concentration with adding of GDNF,the TH positive cells differentiated from NSCs were detected by immunocytochemistry and immunofluorescence methods.Results Low oxygen obviously increased TH expression with significant differences compared to the normal oxygen control group（P<0.01）.What’s more,at 3% low oxygen concentration,there was a highest differentiation rate for NSCs to TH positive neurons.Conclusion Low oxygen concentration promoted the differentiation from NSCs to TH positive neurons.3% oxygen concentration was the optimum low oxygen concentration.There was no dependence relationship between differentiation rate promoted by low oxygen concentration for NSCs to TH positive neurons and oxygen concentration gradient.There was a more significant increase in induction efficiency for combined use of low oxygen and GDNF on induction of differentiation of NSCs into TH positive neurons.
