Dynamic changes of Ephrin-B3 expression in hippocampus of a rat model of temporal lobe epilepsy
10.19845/j.cnki.zfysjjbzz.2021.0189
- VernacularTitle:Ephrin-B3在癫痫大鼠海马中的动态表达变化
- Author:
Tiantian LIU
1
;
Junfang TENG
1
;
Hengfang LIU
1
Author Information
1. Department of Neurology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China
- Publication Type:Journal Article
- Keywords:
Ephrin-B3;
Temporal lobe epilepsy;
Dentate gyrus;
Spontaneous recurrent seizures
- From:
Journal of Apoplexy and Nervous Diseases
2021;38(8):714-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore dynamic expression of Ephrin-B3 in hippocampus of pilocarpine induced epileptic rats and the role of Ephrin-B3 in the process of epileptogenesis.Methods We first established pilocarpine induced epileptic rat model and then detected Ephrin-B3 expression at 7,14 and 60 days post status epilepticus (SE) by real time qPCR,immunohistochemistry and Western blot.Results After pilocarpine injection,rats showed facial muscles,forelimb and tail clonus and ataxic lurching,head bobbing and wet dog shakes.Real time qPCR revealed that Ephrin-B3 mRNA expression in hippocampus decreased during spontaneous seizure period.Compared with controls,epileptic rats showed decreased Ephrin-B3 expression at 7 days,reached the lowest level at 14 days and then increased slightly at 60 days post-SE (P<0.001).Ephrin-B3 protein in hippocampus also decreased at 7,14 and 60 days detected by Western blot (P<0.001).Immunohistochemistry results showed that Ephrin-B3 mainly expressed in granule cells in dentate gyrus.Analysis of immunohistochemistry revealed decreased Ephrin-B3 expression in epileptic rats compared with controls (P<0.001).Conclusion The dynamic changes of Ephrin-B3 expression in hippocampus of epileptic rats indicated that Ephrin-B3 may participate in the process of spontaneous recurrent seizures.
- Full text:20240729195258239321Dynamic changes of Ephrin-B3 expression in hippocampus of a rat model of temporal lobe epilepsy.pdf
