Effects of overexpression of tyrosine kinase receptor 1 on malignant biological behavior of tumor-associated endothelial cells in cervical cancer cells
10.19405/j.cnki.issn1000-1492.2022.11.013
- Author:
Qianyu1 Sun
1
;
Yan1 Wei
1
;
Rui1 Bai
1
;
Ping1 Yang
1
Author Information
1. Dept of Gynecology，The First Affiliated Hospital of Shihezi Medical University，Shihezi 832008 ;Key Laboratory of Prevention and Treatment of Subhigh Incidence Diseases， National Health Commission of China，Shihezi 832008
- Publication Type:Journal Article
- Keywords:
tyrosine kinase receptor 1;
tumor-related endothelial cells;
cervical cancer;
angiogenesis;
co-culture
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1756-1762
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of overexpression of tyrosine kinase receptor 1 (Tie-1) in cervical cancer cells on the malignant biological behavior of tumor-related endothelial cells (TRECs) ．
Methods :Immuno- histochemical method was used to detect the expression of Tie-1 in cervical cancer cells ( CCCs) and TRECs of 96 patients with cervical cancer，and to analyze the correlation between the expression of Tie-1 in TRECs and clinico- pathological features and prognosis of patients．HeLa cells overexpressing Tie-1 (Hela-Tie1OE) were constructed， and HeLa-Tie1OE was co-cultured with human umbilical vein endothelial cells ( HUVECs) by Transwell cell co- culture method to obtain cervical cancer TRECs．Western blot was used to analyze Tie-1 protein expression．The migration，invasion and tubulogenesis of TRECs were detected by cell scratch assay，Transwell invasion and migra- tion assay and tubulogenesis assay.
Results :The expression of Tie-1 was positively correlated with CCCs and TRECs in 96 cervical cancer patients．The positive expression rate of Tie-1 in TRECs of patients with stage(FIGO) Ⅰ B2-ⅡA，tumor diameter ≥4 cm ，cervical muscle invasion depth ≥ 1 /2 full layer ，adenocarcinoma ，medium and low differentiation cervical cancer was higher than that of patients with Ⅰ A 1-Ⅰ B 1，tumor diameter＜4 cm， cervical muscle invasion depth ＜1 /2 full layer，squamous carcinoma，and high differentiation cervical cancer，re- spectively．The differences were statistically significant (P＜0. 05) ．The expression of Tie-1 in TRECs of cervical cancer patients had no significant correlation with age，lymph node metastasis and lymphatic space invasion．The positive expression of Tie-1 in cervical cancer TRECs was negatively correlated with 5-year progression-free survival time and overall survival time (P＜0. 05) ．The Tie-1 expression of TRECs obtained by co-culture of Hela-Tie1OE and HUVECs was up-regulated ，and the invasion ，migration and tubulogenesis of TRECs cells were enhanced.
Conclusion :The high expression of Tie-1 in TRECs of cervical cancer is related to FIGO stage，tumor diameter， degree of differentiation，depth of cervical muscular invasion，type of cervical cancer，and poor prognosis of pa- tients．Overexpression of Tie-1 can promote TRECs invasion，migration and tubulogenesis.
- Full text:2024072521005395810宫颈癌细胞过表达酪氨酸激酶...皮细胞恶性生物学行为的影响_孙倩玉.pdf
