Application and Biological Evaluation of Magnetic Cell Sorting Technology
10.16476/j.pibb.2023.0009
- VernacularTitle:磁性细胞分选技术的应用与生物学评价
- Author:
Tian HONG
1
;
Jing-Wen LI
1
;
Ren-Ai LI
2
;
Er-Ning CHEN
1
;
Lu-Lu ZHAO
1
;
Mei-Hong DU
1
Author Information
1. Institute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical & Chemical Analysis), Bejing 100094, China
2. School of Biomedicine, Beijing City University, Beijing 100094, China
- Publication Type:Journal Article
- Keywords:
magnetic cell sorting;
biological evaluation;
cell sorting technology;
magnetic material;
lymphocyte
- From:
Progress in Biochemistry and Biophysics
2024;51(1):123-135
- CountryChina
- Language:Chinese
-
Abstract:
Magnetic cell sorting technology is a highly specific and rapid cell sorting technology using superparamagnetic nanocomposites for cell sorting, which is widely used in immunology, stem cytology, oncology, clinical medicine and other fields. Magnetic cell sorting technology is divided into positive isolation, negative isolation/untouched cell isolation, depletion, multi-step isolation and automated cell separation systems. In this review, we firstly give a brief introduction to the classification and application of magnetic cell sorting technology, then discuss several new techniques and challenges based on magnetic cell sorting in recent years, such as improving the sorting efficiency by improving the structure of magnetic materials and magnetic field structure. The necessity of biological evaluation of magnetic cell sorting products was emphatically analyzed. Through the biological evaluation, the advantages and disadvantages of magnetic cell sorting products can be understood, and the research and development ability could be improved. Therefore, 10 biological evaluation technical parameters related to magnetic cell sorting products were proposed: yield, purity, sterility, cytotoxicity, cell morphology, viability, light scattering characteristics of cells, fluorescent antibody labeling ability of cells, cell activation and cell proliferation. The 10 biological evaluation technical parameters play an important role in promoting the standardized application of magnetic cell sorting.
