Effect of Combined Frequency Stimulation on The Electrophysiology of Granule Neurons in The Hippocampal Dentate Gyrus Area of Hindlimb Unloading Mice
10.16476/j.pibb.2023.0431
- VernacularTitle:复合频率刺激对后肢去负荷小鼠海马齿状回区颗粒神经元作用的电生理研究
- Author:
Jun-Qiao ZHAO
1
;
Ming-Qiang ZHU
1
;
Hai-Jun ZHU
2
;
Rui FU
1
;
Ze ZHANG
1
;
Jia-Le WANG
1
;
Chong DING
1
Author Information
1. School of Health Sciences & Biomedical Engineering, Hebei University of Technology, Tianjin 300130, China
2. Key Laboratory of Digital Medical Engineering of Hebei Province, Hebei University, Baoding 071002, China
- Publication Type:Journal Article
- Keywords:
combined frequency stimulation;
repetitive transcranial magnetic stimulation;
hindlimb unloading;
spatial cognitive impairment;
negative emotion;
neuronal excitability;
ion channels
- From:
Progress in Biochemistry and Biophysics
2024;51(7):1670-1686
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveIn recent years, the negative impact of microgravity on astronauts’ nervous systems has received widespread attention. The repetitive transcranial magnetic stimulation (rTMS) technology has shown significant positive effects in the treatment of neurological and psychiatric disorders. The potential benefits of combined frequency stimulation (CFS) which combines different frequency stimulation patterns in ameliorating neurological dysfunctions induced by the microgravity environment, still require in-depth investigation. Exploring the therapeutic effects and electrophysiological mechanisms of CFS in improving various neurological disorders caused by microgravity holds significant importance for neuroscience and the clinical application of magnetic stimulation. MethodsThis study employed 40 C57BL/6 mice, randomly divided into 5 groups: sham group, hindlimb unloading (HU) group, 10 Hz group, 20 Hz group, and combined frequency stimulation (10 Hz+20 Hz, CFS) group. Mice in all groups except the sham group received 14 d of simulated microgravity conditions along with 14 d of repetitive transcranial magnetic stimulation. The effects of CFS on negative emotions and spatial cognitive abilities were assessed through sucrose preference tests and water maze experiments. Finally, patch-clamp techniques were used to record action potentials, resting membrane potentials, and ion channel dynamics of granule neurons in the hippocampal dentate gyrus (DG) region. ResultsCompared to the single-frequency stimulation group, behavioral results indicated that the combined frequency stimulation (10 Hz+20 Hz) significantly improved cognitive impairments and negative emotions in simulated microgravity mice. Electrophysiological experiments revealed a decrease in excitability of granule neurons in the hippocampal DG region after HU manipulation, whereas the combined frequency stimulation notably enhanced neuronal excitability and improved the dynamic characteristics of voltage-gated Na+ and K+ channels. ConclusionThe repetitive transcranial magnetic stimulation with combined frequencies (10 Hz+20 Hz) effectively ameliorates cognitive impairments and negative emotions in simulated microgravity mice. This improvement is likely attributed to the influence of combined frequency stimulation on neuronal excitability and the dynamic characteristics of Na+ and K+ channels. Consequently, this study holds the promise to provide a theoretical basis for alleviating cognitive and emotional disorders induced by microgravity environments.
