Sericin ameliorates epithelial －mesenchymal transition of podocytes induced by high glucose
10.19405/j.cnki.issn1000-1492.2022.12.012
- Author:
Ting Ge
1
;
Shuo Shi
2
;
Yuxuan Song
2
;
Hongjiao Ruan
2
;
Zhihong Chen
2
;
Chengjun Song
2
Author Information
1. Dept of Human Anatomy，Chengde Medical University，Chengde 067000;Centralab，Changshu No．1 People 's Hospital，Suzhou 215500
2. Dept of Human Anatomy，Chengde Medical University，Chengde 067000
- Publication Type:Journal Article
- Keywords:
sericin;
diabetic kidney disease;
podocyte;
epithelial-mesenchymal transition
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1915-1921
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effect of sericin on podocyte epithelial-mesenchymal transition induced by high glucose．
Methods :Conditional immortalized mouse podocytes were used as the research object to construct podocyte injury model by high glucose stimulation．The podocytes were divided into normal (NG) group ，hyperton- ic control (MG) group ，high glucose injury ( HG) group ，low ，medium and high dose sericin ( LS ，MS ，HS) group．CCK-8 method was used to determine the viability of podocytes in each group ; the morphological structure of podocytes in each group was observed by ordinary optical inverted microscope．The migration ability was detected by Transwell and cell scratch test．Western blot was used to detect the expression levels of EMT-related mesenchy- mal phenotype factors Snai1，α-SMA，FSP-1，MMP-9 and epithelial phenotype factors Nephrin，E-cadherin，and WT-1 in podocytes of each group.
Results :Compared with NG group，HG group showed lower podocyte activity (P＜0. 01) ，worse podocyte status and enhanced podocyte migration ability，up-regulated Snai1，α-SMA，FSP-1 and MMP-9 protein expressions (P ＜0. 01) ，and down-regulated Nephrin，E-cadherin and WT-1 protein expres- sions (P＜0. 01) ．Compared with HG group，the viability of podocytes in sericin group increased (P＜0. 05) ，the state of podocytes was improved ，the migration ability of podocytes was weakened ，the expression of Snai1 ，α - SMA，FSP-1 and MMP-9 was down-regulated (P＜0. 05) ，and the expression of Nephrin，E-cadherin and WT-1 was up-regulated (P ＜0. 05 ) ．
Conclusion :Sericin can alleviate podocyte epithelial-mesenchymal transition in- duced by high glucose.
- Full text:2024072116185330587丝胶改善高糖诱导的足细胞上皮间质转化_葛婷.pdf
