Intravaginally CpG-ODN and Salmonella enteritidis on TLR21, cytokines, and AvBD10 gene expressions in the reproductive tract of native chicken
https://doi.org/10.47665/tb.41.2.002
- Author:
Suryohastari, R.R.B.
1
;
Sumarsono, S.H.
2
;
Giri-Rachman, E.A.
2
;
Edi, S.P.
3
Author Information
1. School of Life Sciences and Technology, Bandung Institute of Technology, 10 Ganeca Street, Bandung, West Java 40132, Indonesia&Department of Biology, Faculty of Sciences and Technology, Syarif Hidayatullah State Islamic University Jakarta, 95 Ir. H. Juanda Street, Cempaka Putih, East Ciputat, South Tangerang, Banten 15412, Indonesia
2. School of Life Sciences and Technology, Bandung Institute of Technology, 10 Ganeca Street, Bandung, West Java 40132, Indonesia&
3. Subang Veterinary Center, Terusan Garuda Street, Subang, West Java 41212, Indonesia
- Publication Type:Journal Article
- Keywords:
AvBD10;
IL1B;
IL10;
Salmonellosis;
TLR21.
- From:Tropical Biomedicine
2024;41(No.2):142-148
- CountryMalaysia
- Language:English
-
Abstract:
Salmonella enterica subsp. enterica serovar Enteritidis (SE) is a global concern for the poultry industry
due to its association with foodborne illnesses. The transmission occurs through the transovarial route
which initiates from colonization in oviducts and ascending to ovaries. Though there are studies on
cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) and the increase of innate immune
response, there is limited research on the intravaginal treatment using CpG-ODN. Previous studies
have shown that stimulating CpG-ODN can induce the production of antimicrobial peptide avian
beta-defensins (AvBDs) in vaginal cell cultures, there is limited information on the use of intravaginal
treatment to induce the innate immune system, particularly in the Kampung Unggul Balitbangtan
(KUB-1) chickens (Gallus gallus domesticus). This study investigates the impact of intravaginal CpG-ODN
stimulation on the innate immune response in KUB-1 chicken ovaries and oviducts when challenged
to SE. A total of 39 KUB-1 chickens were divided into four groups namely T1 (treated with CpG-ODN,
n=12), T2 (SE group, n=12), T3 (CpG-ODN and SE, n=12), and Control (without CpG-ODN and SE, n=3).
Chickens were observed from day 1 to 4 post-intravaginal (PI) inoculation. The results suggest that
intravaginal CpG-ODN treatment modulates AvBD10 production through toll-like receptor (TLR)21, with
interleukin (IL)1B and IL10 playing reciprocal roles, providing insights into the potential of this treatment
to prevent transovarial Salmonellosis in poultry. The novelty of this study adds valuable insights to the
current body of knowledge.
- Full text:20240717163720769488.2024my1463.pdf
