Effect of CTRP9 on lipid metabolism in brown adipose tissue of mice induced by cold stimulation
10.19405/j.cnki.issn1000-1492.2023.04.010
- Author:
Hua Guan
1
;
Huanhuan Chang
2
;
Xiangyu Li
3
;
Xue Wang
4
;
Yang Gao
4
;
Junjun Hao
4
;
Fengwei Guo
4
;
Tao Shi
4
Author Information
1. Laboratory for Lipid Metabolism and Atherosclerosis，Xi 'an Jiaotong University Cardiovascular Research Center， Xi 'an 710061 ; Institute of Basic and Translational Medicine of Xi 'an Medical University，Xi 'an 710021
2. Dept of Otolaryngology，Xi 'an Children 's Hospital Affiliated to Xi 'an Jiaotong University，Xi 'an 710003
3. Institute of Basic and Translational Medicine of Xi 'an Medical University，Xi 'an 710021
4. Dept of Cardiovascular Surgery，The First Affiliated Hospital of Xi 'an Jiaotong University，Xi 'an 710061
- Publication Type:Journal Article
- Keywords:
cold stimulation;
CTRP9;
brown adipose tissue;
UCP-1;
ATGL
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):577-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of C1q / tumor necrosis factor-related protein 9 ( CTRP9 ) on the expression of genes and proteins related to lipid metabolism of brown adipose tissue (BAT) in mice after cold stimulation．
Methods :C57BL /6J male mice were injected with adenovirus Ad-GFP (control group) or Ad-CTRP9 ( experience group) into the scapular region and kept for 7 days．After cold stimulation at 4 ℃ for 10 hours，the expression levels of BAT marker genes and proteins were detected by real time PCR and Western blot.
Results:Overexpression of CTRP9 induced by cold stimulation significantly increased the mRNA level of iodothyronine deiodinase 2 (Dio2) in BAT (P＜0. 01) ．Additionally，there was no significant difference in the expression of BAT marker genes ( UCP-1，PGC-1 α , PRDM16 and ARβ3) ，and liposynthesis and lipolysis related genes (PPARγ , HSL and ATGL) ．Uncoupling protein 1 (UCP-1) protein expression was upregualted in Ad-CTRP9 compared to the Ad-GFP control group ，while the expression of lipolysis related protein adipose triglyceride lipase ( ATGL) decreased significantly (P＜0. 05) ．
Conclusion:In cold environment，overexpression of CTRP9 promotes the accumulation of UCP-1 protein in BAT，upregulates the expression of thyroid hormone signal related gene Dio2，and inhibits triglyceride hydrolysis to maintain a constant body temperature.
- Full text:2024071710373162036CTRP9对冷刺激诱导的小...棕色脂肪组织脂质代谢的影响_关华.pdf
