Construction of a chimeric antigen receptor CAR THP －1 cell line targeting HER2
- Author:
Yizhao Chen
1
;
Lihua Liu
1
;
Xiangling Zhu
1
;
Huihui Wang
1
;
Xuming Wu
1
;
Xuewen Tan
1
;
Yilong Fang
1
;
Haifeng Jiang
1
;
Zhen Xu
1
;
Wei Wei
1
;
Jiajie Tu
1
Author Information
- Publication Type:Journal Article
- Keywords: chimeric antigen receptor macrophage; tumor; phagocytosis; lentivirus
- From: Acta Universitatis Medicinalis Anhui 2023;58(3):352-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain chimeric antigen receptor macrophages ( CAR-M) targeting HER2 stably transfected．
Methods :CAR lentivirus vector targeting HER2 was constructed and infected with human monocytic leukemia cell line (THP-1) ．CAR THP-1 cells with green fluorescent labeling were selected by sorting flow cytometry and continued to be cultured in vitro．The CAR THP-1 cells targeting HER2 were co-cultured with the endometrial cancer cell line Ishikawa with negative and positive HER2 expression，and their targeted phagocytosis of CAR-M to HER2 positive tumor cells was detected by imaging flow cytometry ，and the targeted phagocytosis efficiency of CAR-M to HER2 positive tumor cells was detected by flow cytometry.
Results :CAR lentivirus infection with THP- 1 cells was less efficient ; After co-culture with cancer cells，flow cytometry and imaging flow cytometry showed that CAR THP-1 cells had enhanced phagocytosis of HER2 positive Ishikawa cells compared with the empty body group (P＜0. 01) ．
Conclusion :In this experiment，CAR THP-1 cell line targeting HER2 was established by constructing CAR lentivirus vector and transfecting THP-1 cells ，and it was proved that CAR THP-1 could phagocytize HER2 positive Ishikawa cells through specific targeting. - Full text:2024071610492333865一种靶向HER2嵌合抗原受...R_THP-1细胞系的构建_陈义昭.pdf
