Mechanism study of interfered NFATc1 expression suppressing cell cycle progression in colorectal cancer cell
10.19405/j.cnki.issn1000-1492.2023.10.016
- Author:
Guangyao Xu
1
;
Can Huang
1
Author Information
1. Dept of Biochemistry and Molecular Biology，School of Basic Medicine， Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
NFATc1;
colon cancer;
cell cycle;
cell proliferation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(10):1701-1706
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Based on the expression of NFATc1 and prognosis in colorectal cancer，we interfered NFATc1 via shRNA transfection，analyzed the effect of NFATc1 on colorectal cancer cell proliferation，and further explored potential mechanisms．
Methods : We explored the correlation between NFATc1 expression and the progno- sis of colorectal cancer using the TCGA database．Thereafter，we compared the expression of NFATc1 in colorectal cancer tissues and adjacent normal tissues by immunohistochemical staining of postoperative samples from clinical colorectal cancer patients．We analyzed the effect of NFATc1 on colorectal cancer cell proliferation by measuring CCK-8 after NFATc1 interfered using shRNA transfection ; Tumorigenic potential of colorectal cancer cells was meas- ured with clone formation assay ; Cell cycle distribution was measured by flow cytometry after propidium iodide staining．The effect of NFATc1 on the transcriptional activity of cell-cycle-related factors was measured by qPCR.
Results : Based on the TCGA data，we found that high NFATc1 expression in colorectal cancer patients was associated with poor prognosis．The expression of NFATc1 in clinical colorectal cancer tissues was significantly higher than that in adjacent normal tissues．Additionally，interference with NFATc1 inhibited the proliferation rate of colorectal canc- er cells in vitro，and the clonogenic capacity of cells was impaired．As expected，the cell cycle was arrested at the G0 / G1 phase．The qPCR results indicated that the knockdown of NFATc1 increased the transcriptional activity of multiple key cell cycle inhibitors．
Conclusion : NFATc1 promotes cell cycle progression by inhibiting the transcrip- tional activity of cell cycle regulatory factors，thereby promoting the proliferation and tumorigenic ability of colorectal cancer cells.
- Full text:2024071218495800726干涉NFATc1表达影响细...转抑制结肠癌细胞增殖的机制_徐光瑶.pdf
